Fluorescence lifetime image of a single halobacterium

Hui Ping Wang; Takakazu Nakabayashi; Kazuo Tsujimoto; Seiji Miyauchi; Naoki Kamo; Nobuhiro Ohta

doi:10.1016/j.cplett.2007.06.034

Fluorescence lifetime image of a single halobacterium

Hui Ping Wang, Takakazu Nakabayashi, Kazuo Tsujimoto, Seiji Miyauchi, Naoki Kamo, Nobuhiro Ohta

PHA - Molecular Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Fluorescence lifetime imaging (FLIM) has been reported for a single halobacterium loaded with 2′,7′-bis-(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). FLIM of halobacteria exhibits two distinct fluorescence lifetimes, representing different environments from each other in different halobacteria. The observed difference in the fluorescence lifetime appears to be due to the difference in electric field inside a cell. Fluorescence intensity of BCECF in polyvinyl alcohol film is quenched by an external electric field, indicating that the fluorescence lifetime of BCECF is affected by an electric field.

Original language	English
Pages (from-to)	441-444
Number of pages	4
Journal	Chemical Physics Letters
Volume	442
Issue number	4-6
DOIs	https://doi.org/10.1016/j.cplett.2007.06.034
Publication status	Published - 2007 Jul 17

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10.1016/j.cplett.2007.06.034

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title = "Fluorescence lifetime image of a single halobacterium",

abstract = "Fluorescence lifetime imaging (FLIM) has been reported for a single halobacterium loaded with 2′,7′-bis-(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). FLIM of halobacteria exhibits two distinct fluorescence lifetimes, representing different environments from each other in different halobacteria. The observed difference in the fluorescence lifetime appears to be due to the difference in electric field inside a cell. Fluorescence intensity of BCECF in polyvinyl alcohol film is quenched by an external electric field, indicating that the fluorescence lifetime of BCECF is affected by an electric field.",

author = "Wang, {Hui Ping} and Takakazu Nakabayashi and Kazuo Tsujimoto and Seiji Miyauchi and Naoki Kamo and Nobuhiro Ohta",

note = "Funding Information: This work has been supported by a Grant-in-Aid for Scientific Research in Priority Area {\textquoteleft}Molecular Nano Dynamics{\textquoteright} from the Ministry of Education, Culture, Sports, Science, and Technology in Japan.",

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doi = "10.1016/j.cplett.2007.06.034",

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T1 - Fluorescence lifetime image of a single halobacterium

AU - Wang, Hui Ping

AU - Nakabayashi, Takakazu

AU - Tsujimoto, Kazuo

AU - Miyauchi, Seiji

AU - Kamo, Naoki

AU - Ohta, Nobuhiro

N1 - Funding Information: This work has been supported by a Grant-in-Aid for Scientific Research in Priority Area ‘Molecular Nano Dynamics’ from the Ministry of Education, Culture, Sports, Science, and Technology in Japan.

PY - 2007/7/17

Y1 - 2007/7/17

N2 - Fluorescence lifetime imaging (FLIM) has been reported for a single halobacterium loaded with 2′,7′-bis-(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). FLIM of halobacteria exhibits two distinct fluorescence lifetimes, representing different environments from each other in different halobacteria. The observed difference in the fluorescence lifetime appears to be due to the difference in electric field inside a cell. Fluorescence intensity of BCECF in polyvinyl alcohol film is quenched by an external electric field, indicating that the fluorescence lifetime of BCECF is affected by an electric field.

AB - Fluorescence lifetime imaging (FLIM) has been reported for a single halobacterium loaded with 2′,7′-bis-(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). FLIM of halobacteria exhibits two distinct fluorescence lifetimes, representing different environments from each other in different halobacteria. The observed difference in the fluorescence lifetime appears to be due to the difference in electric field inside a cell. Fluorescence intensity of BCECF in polyvinyl alcohol film is quenched by an external electric field, indicating that the fluorescence lifetime of BCECF is affected by an electric field.

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JO - Chemical Physics Letters

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Fluorescence lifetime image of a single halobacterium

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