TY - JOUR
T1 - Fluoridated Ca-deficient apatite formed via octacalcium phosphate enhances bone regeneration
AU - Kamakura, Shinji
AU - Kumagai, Takashi
AU - Honda, Yoshitomo
AU - Anada, Takahisa
AU - Sasaki, Keiichi
AU - Shimauchi, Hidetoshi
AU - Suzuki, Osamu
PY - 2006
Y1 - 2006
N2 - It has been shown that fluoride ions enhance OCP hydrolysis into Ca-deficient apatite and that fluoridation in hydroxyapatite (HA) affects osteoblast activity. The present study was designed to investigate whether fluoridated Ca-deficient apatite (F-HA) formed via OCP enhances bone regeneration. F-HA was obtained through hydrolysis of the OCP in a solution containing 2 ppm fluoride at 37°C and pH 7.4. A standardized critical-sized defect was made in the rat calvarium, and granules of F-HA were implanted into the defect. Five rats from each group were fixed through four to twelve weeks after implantation. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) confirmed that F-HA corresponded well to apatite structure. In week four, new bone matrix was formed around F-HA. In week twelve of F-HA group, newly formed bone matrix was more abundant, whereas the implanted F-HA was unresorbed and still remained. A statistical analysis in week twelve showed that the newly formed bone in the defect with F-HA was higher than that with untreated group. The fact that new bone was directly formed on F-HA implant suggests F-HA formed via OCP could be used as a bone substitute material.
AB - It has been shown that fluoride ions enhance OCP hydrolysis into Ca-deficient apatite and that fluoridation in hydroxyapatite (HA) affects osteoblast activity. The present study was designed to investigate whether fluoridated Ca-deficient apatite (F-HA) formed via OCP enhances bone regeneration. F-HA was obtained through hydrolysis of the OCP in a solution containing 2 ppm fluoride at 37°C and pH 7.4. A standardized critical-sized defect was made in the rat calvarium, and granules of F-HA were implanted into the defect. Five rats from each group were fixed through four to twelve weeks after implantation. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) confirmed that F-HA corresponded well to apatite structure. In week four, new bone matrix was formed around F-HA. In week twelve of F-HA group, newly formed bone matrix was more abundant, whereas the implanted F-HA was unresorbed and still remained. A statistical analysis in week twelve showed that the newly formed bone in the defect with F-HA was higher than that with untreated group. The fact that new bone was directly formed on F-HA implant suggests F-HA formed via OCP could be used as a bone substitute material.
KW - Bone regeneration
KW - Ca-deficient apatite
KW - Octacalcium phosphate
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U2 - 10.4028/0-87849-992-x.137
DO - 10.4028/0-87849-992-x.137
M3 - Article
AN - SCOPUS:33645384027
SN - 1013-9826
VL - 309-311 I
SP - 137
EP - 140
JO - Key Engineering Materials
JF - Key Engineering Materials
ER -