Fluorogenic protein labeling through photoinduced electron transfer-based BL-tag technology

Kalyan K. Sadhu; Shin Mizukami; Carolyn R. Lanam; Kazuya Kikuchi

doi:10.1002/asia.201100647

Fluorogenic protein labeling through photoinduced electron transfer-based BL-tag technology

Kalyan K. Sadhu, Shin Mizukami, Carolyn R. Lanam, Kazuya Kikuchi

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Keeping quenchers on a short leash: Shortening of the linker chain length combined with modification of the quencher moiety was found to improve fluorogenic probes useful for protein labeling by the mutant β-lactamase-tag (BL-tag) technology. The most effective probe, CC3 DNB, displays fluorescence quenching by photoinduced electron transfer (PET) and results in fluorescent protein labeling with fast kinetics.

Original language	English
Pages (from-to)	272-276
Number of pages	5
Journal	Chemistry - An Asian Journal
Volume	7
Issue number	2
DOIs	https://doi.org/10.1002/asia.201100647
Publication status	Published - 2012 Feb 6
Externally published	Yes

Keywords

biotechnology
electron transfer
fluorescence spectroscopy
fluorescent probesprotein labeling

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

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10.1002/asia.201100647

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abstract = "Keeping quenchers on a short leash: Shortening of the linker chain length combined with modification of the quencher moiety was found to improve fluorogenic probes useful for protein labeling by the mutant β-lactamase-tag (BL-tag) technology. The most effective probe, CC3 DNB, displays fluorescence quenching by photoinduced electron transfer (PET) and results in fluorescent protein labeling with fast kinetics.",

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author = "Sadhu, {Kalyan K.} and Shin Mizukami and Lanam, {Carolyn R.} and Kazuya Kikuchi",

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AU - Mizukami, Shin

AU - Lanam, Carolyn R.

AU - Kikuchi, Kazuya

PY - 2012/2/6

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AB - Keeping quenchers on a short leash: Shortening of the linker chain length combined with modification of the quencher moiety was found to improve fluorogenic probes useful for protein labeling by the mutant β-lactamase-tag (BL-tag) technology. The most effective probe, CC3 DNB, displays fluorescence quenching by photoinduced electron transfer (PET) and results in fluorescent protein labeling with fast kinetics.

KW - biotechnology

KW - electron transfer

KW - fluorescence spectroscopy

KW - fluorescent probesprotein labeling

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Fluorogenic protein labeling through photoinduced electron transfer-based BL-tag technology

Abstract

Keywords

ASJC Scopus subject areas

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