Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH

Hiroshi Maeda; Tomohiro Sawa; Toshitugu Yubisui; Takaaki Akaike

doi:10.1016/S0304-3835(99)00139-1

Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH

Hiroshi Maeda, Tomohiro Sawa, Toshitugu Yubisui, Takaaki Akaike

MED - Public Health

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

We previously reported findings that NADPH/cytochrome P450 reductase can generate superoxide anion radical (O₂(^.-)) from heterocyclic amines (HCA) and from many anticancer agents in vitro. Here we present more evidence in which O₂(^.-) is generated when recombinant human cytochrome b5 reductase (rh-Cytb5Rd) was incubated with HCAs such as IQ and MeIQ in the presence of NADH in vitro. This indicates that free radical generation by rh-Cytb5Rd in the presence of HCA may add new insight into the damage of DNA in addition to the previously known mechanism: interaction of activated HCA-intermediates to form DNA adduct. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	117-121
Number of pages	5
Journal	Cancer Letters
Volume	143
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3835(99)00139-1
Publication status	Published - 1999 Sept

Keywords

Cytochrome b5 reductase
DNA damage
Electron spin resonance
Heterocyclic amines
Superoxide generation

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10.1016/S0304-3835(99)00139-1

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title = "Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH",

abstract = "We previously reported findings that NADPH/cytochrome P450 reductase can generate superoxide anion radical (O2(.-)) from heterocyclic amines (HCA) and from many anticancer agents in vitro. Here we present more evidence in which O2(.-) is generated when recombinant human cytochrome b5 reductase (rh-Cytb5Rd) was incubated with HCAs such as IQ and MeIQ in the presence of NADH in vitro. This indicates that free radical generation by rh-Cytb5Rd in the presence of HCA may add new insight into the damage of DNA in addition to the previously known mechanism: interaction of activated HCA-intermediates to form DNA adduct. Copyright (C) 1999 Elsevier Science Ireland Ltd.",

keywords = "Cytochrome b5 reductase, DNA damage, Electron spin resonance, Heterocyclic amines, Superoxide generation",

author = "Hiroshi Maeda and Tomohiro Sawa and Toshitugu Yubisui and Takaaki Akaike",

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T1 - Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH

AU - Maeda, Hiroshi

AU - Sawa, Tomohiro

AU - Yubisui, Toshitugu

AU - Akaike, Takaaki

PY - 1999/9

Y1 - 1999/9

N2 - We previously reported findings that NADPH/cytochrome P450 reductase can generate superoxide anion radical (O2(.-)) from heterocyclic amines (HCA) and from many anticancer agents in vitro. Here we present more evidence in which O2(.-) is generated when recombinant human cytochrome b5 reductase (rh-Cytb5Rd) was incubated with HCAs such as IQ and MeIQ in the presence of NADH in vitro. This indicates that free radical generation by rh-Cytb5Rd in the presence of HCA may add new insight into the damage of DNA in addition to the previously known mechanism: interaction of activated HCA-intermediates to form DNA adduct. Copyright (C) 1999 Elsevier Science Ireland Ltd.

AB - We previously reported findings that NADPH/cytochrome P450 reductase can generate superoxide anion radical (O2(.-)) from heterocyclic amines (HCA) and from many anticancer agents in vitro. Here we present more evidence in which O2(.-) is generated when recombinant human cytochrome b5 reductase (rh-Cytb5Rd) was incubated with HCAs such as IQ and MeIQ in the presence of NADH in vitro. This indicates that free radical generation by rh-Cytb5Rd in the presence of HCA may add new insight into the damage of DNA in addition to the previously known mechanism: interaction of activated HCA-intermediates to form DNA adduct. Copyright (C) 1999 Elsevier Science Ireland Ltd.

KW - Cytochrome b5 reductase

KW - DNA damage

KW - Electron spin resonance

KW - Heterocyclic amines

KW - Superoxide generation

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VL - 143

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EP - 121

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH

Abstract

Keywords

UN SDGs

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