@article{335dc956786e4ac283916999e1b3771f,
title = "Frequent asymptomatic infection with tobacco ringspot virus on melon fruit",
abstract = "Melon is one of the most popular fruits worldwide and has been bred into various cultivars. RNA-sequencing using healthy melon fruit was performed to determine differences in gene expression among cultivars. Unexpected RNA-seq results revealed that viruses asymptomatically infected fruits at a high frequency (16 of 21 fruits examined were infected) and that viral transcripts highly accumulated in comparison with host transcripts (15 %–75 % of total reads). Their nucleotide sequences and phylogenetic analyses indicated that more than 10 novel isolates of tobacco ringspot virus (TRSV) were found in melon fruits. Asymptomatic infection with TRSV on melon fruits was confirmed by both immunoblot and RT-PCR analyses. Numerous isolates of TRSV generated and maintained in melon fields, and this is likely due to their asymptomatic infections. This TRSV melon isolate infected Nicotiana benthamiana plants with stunting and yellowing symptoms. This is the first report of frequent and asymptomatic infection of TRSV in consumable melon fruits.",
keywords = "Asymptomatic infection, Cucumis melo, Melon, RNA-seq, Tobacco ringspot virus",
author = "Midori Tabara and Yukihiro Nagashima and Kai He and Xiaoning Qian and Crosby, {Kevin M.} and John Jifon and Jayaprakasha, {G. K.} and Bhimanagouda Patil and Hisashi Koiwa and Hideki Takahashi and Toshiyuki Fukuhara",
note = "Funding Information: We would like to thank Ehsan Hajiramezanali (Texas A&M Electrical and Computer Engineering Department) for his assistance on initial RNA-seq data analysis. We also thank Mr. Jashbir Shingh and Ms. Rita Metrani for harvesting and processing melon samples used for this study. This project was supported by the USDA-SCRI competitive grant 2017-51181-26834 [to B.P.]. It has been supported in part by U.S. National Science Foundation awards CCF-1553281 and IIS-181264 [to X.Q.]. This work was also supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Scientific Research on Innovative Areas [No. 16H06435, No. 16H06429 and 16H21723] to H.T and T.F. and [No. 19K23674] to M.T.), the Global Innovation Research (GIR) Organization of Tokyo University of Agriculture and Technology [to M.T and T.F.], and the Japan Society for the Promotion of Science (JSPS) through the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety” [to H.T.]. Funding Information: We would like to thank Ehsan Hajiramezanali (Texas A&M Electrical and Computer Engineering Department) for his assistance on initial RNA-seq data analysis. We also thank Mr. Jashbir Shingh and Ms. Rita Metrani for harvesting and processing melon samples used for this study. This project was supported by the USDA-SCRI competitive grant 2017-51181-26834 [to B.P.]. It has been supported in part by U.S. National Science Foundation awards CCF-1553281 and IIS-181264 [to X.Q.]. This work was also supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Scientific Research on Innovative Areas [No. 16H06435 , No. 16H06429 and 16H21723 ] to H.T and T.F. and [No. 19K23674 ] to M.T.), the Global Innovation Research (GIR) Organization of Tokyo University of Agriculture and Technology [to M.T and T.F.], and the Japan Society for the Promotion of Science (JSPS) through the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety” [to H.T.]. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",
year = "2021",
month = feb,
doi = "10.1016/j.virusres.2020.198266",
language = "English",
volume = "293",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
}
