Functional and morphological analysis of rehydrated lyophilized human platelets

T. A. Takahashi; K. Nakai; N. Sato; S. Fujikawa; K. Tadokoro; T. Jyuji; M. Murata; Y. Ikeda

Functional and morphological analysis of rehydrated lyophilized human platelets

T. A. Takahashi, K. Nakai, N. Sato, S. Fujikawa, K. Tadokoro, T. Jyuji, M. Murata, Y. Ikeda

Research output: Contribution to journal › Article › peer-review

Abstract

We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

Original language	English
Pages (from-to)	637-640
Number of pages	4
Journal	Japanese Journal of Artificial Organs
Volume	26
Issue number	3
Publication status	Published - 1997
Externally published	Yes

Keywords

Blood substitutes
Glycoprotein 1b
Lyophilization
Platelets
Ristocetin- dependent aggregation

ASJC Scopus subject areas

Biophysics

Cite this

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title = "Functional and morphological analysis of rehydrated lyophilized human platelets",

abstract = "We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.",

keywords = "Blood substitutes, Glycoprotein 1b, Lyophilization, Platelets, Ristocetin- dependent aggregation",

author = "Takahashi, {T. A.} and K. Nakai and N. Sato and S. Fujikawa and K. Tadokoro and T. Jyuji and M. Murata and Y. Ikeda",

year = "1997",

language = "English",

volume = "26",

pages = "637--640",

journal = "Japanese Journal of Artificial Organs",

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publisher = "Japanese Society for Artificial Organs and Tissues",

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TY - JOUR

T1 - Functional and morphological analysis of rehydrated lyophilized human platelets

AU - Takahashi, T. A.

AU - Nakai, K.

AU - Sato, N.

AU - Fujikawa, S.

AU - Tadokoro, K.

AU - Jyuji, T.

AU - Murata, M.

AU - Ikeda, Y.

PY - 1997

Y1 - 1997

N2 - We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

AB - We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

KW - Blood substitutes

KW - Glycoprotein 1b

KW - Lyophilization

KW - Platelets

KW - Ristocetin- dependent aggregation

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M3 - Article

AN - SCOPUS:0030915083

SN - 0300-0818

VL - 26

SP - 637

EP - 640

JO - Japanese Journal of Artificial Organs

JF - Japanese Journal of Artificial Organs

IS - 3

ER -

Functional and morphological analysis of rehydrated lyophilized human platelets

Abstract

Keywords

ASJC Scopus subject areas

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