TY - JOUR
T1 - Functional assay of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6 (ARF6), in dendritic formation of hippocampal neurons
AU - Sakagami, Hiroyuki
AU - Kamata, Akifumi
AU - Fukunaga, Kohji
AU - Kondo, Hisatake
N1 - Funding Information:
The authors would like to thank Dr. J. Miyazaki (Osaka University Medical School) for kindly providing pCAGGS vector and Dr. A. Miyawaki (RIKEN) for pCS2‐Venus vector. This work is supported by Grants‐in‐Aid for Young Scientists (#15700269) and for Scientific Research on Priority Areas (#16015215) to H.S. from the Ministry of Education, Science, Sports, Culture and Technology of Japan.
PY - 2005
Y1 - 2005
N2 - EFA6A is a guanine nucleotide exchange factor (GEF) that can activate ADP-ribosylation factor 6 (ARF6) in vitro, with prominent expression in the forebrain including the hippocampal formation. In this section, we describe the neuronal transfection method and show that the overexpression of a catalytically inactive mutant of EFA6A induces a prominent dendritic formation of the primary hippocampal neurons, suggesting the intimate involvement of EFA6A in the regulation of neuronal dendritic development. This reliable and consistent neuronal transfection method will also be applicable for the vector-based RNA interference method.
AB - EFA6A is a guanine nucleotide exchange factor (GEF) that can activate ADP-ribosylation factor 6 (ARF6) in vitro, with prominent expression in the forebrain including the hippocampal formation. In this section, we describe the neuronal transfection method and show that the overexpression of a catalytically inactive mutant of EFA6A induces a prominent dendritic formation of the primary hippocampal neurons, suggesting the intimate involvement of EFA6A in the regulation of neuronal dendritic development. This reliable and consistent neuronal transfection method will also be applicable for the vector-based RNA interference method.
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U2 - 10.1016/S0076-6879(05)04022-X
DO - 10.1016/S0076-6879(05)04022-X
M3 - Review article
C2 - 16413273
AN - SCOPUS:30544440802
SN - 0076-6879
VL - 404
SP - 232
EP - 242
JO - Methods in Enzymology
JF - Methods in Enzymology
M1 - 22
ER -