Functional divergence of MYB-related genes, WEREWOLF and AtMYB23 in Arabidopsis

Rumi Tominaga-Wada; Yuka Nukumizu; Shusei Sato; Tomohiko Kato; Satoshi Tabata; Takuji Wada

doi:10.1271/bbb.110811

Functional divergence of MYB-related genes, WEREWOLF and AtMYB23 in Arabidopsis

Rumi Tominaga-Wada, Yuka Nukumizu, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Takuji Wada

LIF - Ecological Developmental Adaptability Life Sciences

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Epidermal cell differentiation in Arabidopsis is studied as a model system to understand the mechanisms that determine the developmental end state of plant cells. MYB-related transcription factors are involved in cell fate determination. To examine the molecular basis of this process, we analyzed the functional relationship of two R2R3-type MYB genes, AtMYB23 (MYB23) and WEREWOLF (WER). MYB23 is involved in leaf trichome formation. WER represses root-hair formation. Swapping domains between MYB23 and WER, we found that a low homology region of MYB23 might be involved in ectopic trichome initiation on hypocotyls. MYB23 and all MYB23-WER (MW) chimeric transgenes rescued the increased roothair phenotype of the wer-1 mutant. Although WER did not rescue the gl1-1 no-trichome phenotype, MYB23 and all MW chimeric transgenes rescued gl1-1. These results suggest that MYB23 acquired a specific function for trichome differentiation during evolution.

Original language	English
Pages (from-to)	883-887
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	76
Issue number	5
DOIs	https://doi.org/10.1271/bbb.110811
Publication status	Published - 2012

Keywords

Arabidopsis thaliana
AtMYB23
Root-hair
Trichome
WEREWOLF

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title = "Functional divergence of MYB-related genes, WEREWOLF and AtMYB23 in Arabidopsis",

abstract = "Epidermal cell differentiation in Arabidopsis is studied as a model system to understand the mechanisms that determine the developmental end state of plant cells. MYB-related transcription factors are involved in cell fate determination. To examine the molecular basis of this process, we analyzed the functional relationship of two R2R3-type MYB genes, AtMYB23 (MYB23) and WEREWOLF (WER). MYB23 is involved in leaf trichome formation. WER represses root-hair formation. Swapping domains between MYB23 and WER, we found that a low homology region of MYB23 might be involved in ectopic trichome initiation on hypocotyls. MYB23 and all MYB23-WER (MW) chimeric transgenes rescued the increased roothair phenotype of the wer-1 mutant. Although WER did not rescue the gl1-1 no-trichome phenotype, MYB23 and all MW chimeric transgenes rescued gl1-1. These results suggest that MYB23 acquired a specific function for trichome differentiation during evolution.",

keywords = "Arabidopsis thaliana, AtMYB23, Root-hair, Trichome, WEREWOLF",

author = "Rumi Tominaga-Wada and Yuka Nukumizu and Shusei Sato and Tomohiko Kato and Satoshi Tabata and Takuji Wada",

note = "Funding Information: We thank Dr. Tetsuya Ishida, Dr. Ryosuke Sano, and Dr. Tetsuya Kurata for useful suggestions. This work was supported financially by the Program to Disseminate the Tenure Tracking System of the Japanese Ministry of Education, Culture, Sports, Science, and Technology, and by a grant for Scientific Research on Priority Areas from the University of Miyazaki.",

year = "2012",

doi = "10.1271/bbb.110811",

language = "English",

volume = "76",

pages = "883--887",

journal = "Bioscience, Biotechnology and Biochemistry",

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AU - Tominaga-Wada, Rumi

AU - Nukumizu, Yuka

AU - Sato, Shusei

AU - Kato, Tomohiko

AU - Tabata, Satoshi

AU - Wada, Takuji

N1 - Funding Information: We thank Dr. Tetsuya Ishida, Dr. Ryosuke Sano, and Dr. Tetsuya Kurata for useful suggestions. This work was supported financially by the Program to Disseminate the Tenure Tracking System of the Japanese Ministry of Education, Culture, Sports, Science, and Technology, and by a grant for Scientific Research on Priority Areas from the University of Miyazaki.

PY - 2012

Y1 - 2012

N2 - Epidermal cell differentiation in Arabidopsis is studied as a model system to understand the mechanisms that determine the developmental end state of plant cells. MYB-related transcription factors are involved in cell fate determination. To examine the molecular basis of this process, we analyzed the functional relationship of two R2R3-type MYB genes, AtMYB23 (MYB23) and WEREWOLF (WER). MYB23 is involved in leaf trichome formation. WER represses root-hair formation. Swapping domains between MYB23 and WER, we found that a low homology region of MYB23 might be involved in ectopic trichome initiation on hypocotyls. MYB23 and all MYB23-WER (MW) chimeric transgenes rescued the increased roothair phenotype of the wer-1 mutant. Although WER did not rescue the gl1-1 no-trichome phenotype, MYB23 and all MW chimeric transgenes rescued gl1-1. These results suggest that MYB23 acquired a specific function for trichome differentiation during evolution.

AB - Epidermal cell differentiation in Arabidopsis is studied as a model system to understand the mechanisms that determine the developmental end state of plant cells. MYB-related transcription factors are involved in cell fate determination. To examine the molecular basis of this process, we analyzed the functional relationship of two R2R3-type MYB genes, AtMYB23 (MYB23) and WEREWOLF (WER). MYB23 is involved in leaf trichome formation. WER represses root-hair formation. Swapping domains between MYB23 and WER, we found that a low homology region of MYB23 might be involved in ectopic trichome initiation on hypocotyls. MYB23 and all MYB23-WER (MW) chimeric transgenes rescued the increased roothair phenotype of the wer-1 mutant. Although WER did not rescue the gl1-1 no-trichome phenotype, MYB23 and all MW chimeric transgenes rescued gl1-1. These results suggest that MYB23 acquired a specific function for trichome differentiation during evolution.

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KW - WEREWOLF

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Functional divergence of MYB-related genes, WEREWOLF and AtMYB23 in Arabidopsis

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