TY - JOUR
T1 - Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy
AU - Oshima, Masamitsu
AU - Inoue, Kaoru
AU - Nakajima, Kei
AU - Tachikawa, Tetsuhiko
AU - Yamazaki, Hiromichi
AU - Isobe, Tomohide
AU - Sugawara, Ayaka
AU - Ogawa, Miho
AU - Tanaka, Chie
AU - Saito, Masahiro
AU - Kasugai, Shohei
AU - Takano-Yamamoto, Teruko
AU - Inoue, Takashi
AU - Tezuka, Katsunari
AU - Kuboki, Takuo
AU - Yamaguchi, Akira
AU - Tsuji, Takashi
N1 - Funding Information:
We are grateful to Y. Shin and T. Takasu (Yamahachi Dental Mfg., Co., Aichi, Japan) for the hydroxyapatite coating of the dental implant. We also thank Y. Ochiai, N. Yamamoto and R. Koitabashi for their technical assistance. This work was supported by Organ Technologies Inc. We also received partial support from the Health and Labour Sciences Research Grants program from the Ministry of Health, Labour, and Welfare (No.21040101) to A.Y. (Tokyo Medical and Dental University) and a Grant-in-Aid for Scientific Research (A) from MEXT, Medicine, Dentistry and Pharmacy (No. 20249078) to T. Tsuji (2008–2010) from the Ministry of Education, Culture, Sports and Technology, Japan.
PY - 2014/8/13
Y1 - 2014/8/13
N2 - Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy.
AB - Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy.
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U2 - 10.1038/srep06044
DO - 10.1038/srep06044
M3 - Article
C2 - 25116435
AN - SCOPUS:84906071047
SN - 2045-2322
VL - 4
JO - Scientific Reports
JF - Scientific Reports
M1 - 6044
ER -