FUT-175 as a potent inhibitor of C5/C3 convertase activity for production of C5a and C3a

Reiko Inagi; Toshio Miyata; Kenji Maeda; Satoshi Sugiyama; Akio Miyama; Izumi Nakashima

doi:10.1016/0165-2478(91)90243-4

FUT-175 as a potent inhibitor of C5/C3 convertase activity for production of C5a and C3a

Reiko Inagi, Toshio Miyata, Kenji Maeda, Satoshi Sugiyama, Akio Miyama, Izumi Nakashima

MED - United Centers for Advanced Research and Translational Medicine (ART)

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

We examined the inhibitory effect of FUT-175 on the C3/C5 convertase activity of the cobra venom factor-derived enzyme CVF,Bb by measuring C5b6-mediated reactive lysis of unsensitized guinea pig erythrocytes and by measuring directly the released fragments C3-des-Arg and C5a-des-Arg. In this study, we showed that the concentration of 4.5 × 10^-6 M of FUT-175 caused 50% inhibition of C5 convertase activity of CVF,Bb in reactive hemolysis assays, and that 4.0×10^-6 M FUT-175 caused 50% inhibition of the production of C3a and C5a generated by the C3/C5 convertase activity of CVF,Bb.

Original language	English
Pages (from-to)	49-52
Number of pages	4
Journal	Immunology Letters
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/0165-2478(91)90243-4
Publication status	Published - 1991 Jan

Keywords

C3/C5 convertase
C3a-des-Arg
C5a-des-Arg
CVF,Bb
Protease inhibitor

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10.1016/0165-2478(91)90243-4

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title = "FUT-175 as a potent inhibitor of C5/C3 convertase activity for production of C5a and C3a",

abstract = "We examined the inhibitory effect of FUT-175 on the C3/C5 convertase activity of the cobra venom factor-derived enzyme CVF,Bb by measuring C5b6-mediated reactive lysis of unsensitized guinea pig erythrocytes and by measuring directly the released fragments C3-des-Arg and C5a-des-Arg. In this study, we showed that the concentration of 4.5 × 10-6 M of FUT-175 caused 50% inhibition of C5 convertase activity of CVF,Bb in reactive hemolysis assays, and that 4.0×10-6 M FUT-175 caused 50% inhibition of the production of C3a and C5a generated by the C3/C5 convertase activity of CVF,Bb.",

keywords = "C3/C5 convertase, C3a-des-Arg, C5a-des-Arg, CVF,Bb, Protease inhibitor",

author = "Reiko Inagi and Toshio Miyata and Kenji Maeda and Satoshi Sugiyama and Akio Miyama and Izumi Nakashima",

year = "1991",

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T1 - FUT-175 as a potent inhibitor of C5/C3 convertase activity for production of C5a and C3a

AU - Inagi, Reiko

AU - Miyata, Toshio

AU - Maeda, Kenji

AU - Sugiyama, Satoshi

AU - Miyama, Akio

AU - Nakashima, Izumi

PY - 1991/1

Y1 - 1991/1

N2 - We examined the inhibitory effect of FUT-175 on the C3/C5 convertase activity of the cobra venom factor-derived enzyme CVF,Bb by measuring C5b6-mediated reactive lysis of unsensitized guinea pig erythrocytes and by measuring directly the released fragments C3-des-Arg and C5a-des-Arg. In this study, we showed that the concentration of 4.5 × 10-6 M of FUT-175 caused 50% inhibition of C5 convertase activity of CVF,Bb in reactive hemolysis assays, and that 4.0×10-6 M FUT-175 caused 50% inhibition of the production of C3a and C5a generated by the C3/C5 convertase activity of CVF,Bb.

AB - We examined the inhibitory effect of FUT-175 on the C3/C5 convertase activity of the cobra venom factor-derived enzyme CVF,Bb by measuring C5b6-mediated reactive lysis of unsensitized guinea pig erythrocytes and by measuring directly the released fragments C3-des-Arg and C5a-des-Arg. In this study, we showed that the concentration of 4.5 × 10-6 M of FUT-175 caused 50% inhibition of C5 convertase activity of CVF,Bb in reactive hemolysis assays, and that 4.0×10-6 M FUT-175 caused 50% inhibition of the production of C3a and C5a generated by the C3/C5 convertase activity of CVF,Bb.

KW - C3/C5 convertase

KW - C3a-des-Arg

KW - C5a-des-Arg

KW - CVF,Bb

KW - Protease inhibitor

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JO - Immunology Letters

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ER -

FUT-175 as a potent inhibitor of C5/C3 convertase activity for production of C5a and C3a

Abstract

Keywords

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