Gel sheet based skeletal muscle cell culture system integrated with the microelectrode array device

We developed the fibrin gel-based C₂C₁₂ myotube culture system integrated with the microelectrode array device to afford a physiologically relevant and stable bioassay system. Myotube/fibrin gel culture system was prepared by transferring a myotube monolayer from a glass substrate to a fibrin gel while retaining the original line patterns of myotubes. By attaching the myotube/fibrin gel onto the microelectrode array, we demonstrated site-specific electrical pulse stimulation of each myotube line pattern. This device would enable highly-accurate bioassay of contraction-mediated glucose metabolic alterations in myotubes by patterning test and control cells stimulated with different contraction/exercise stress next to each other.