Gene expression analysis with DNA array technology in legumes

Masao Watanabe, Makoto Endo, Hirokazu Hakozaki, Hiromi Masuko, Jong In Park, Makiko Kawagishi-Kobayashi, Tohru Tsuchiya

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


The family Fabaceae is highly divergent at the species level and comprises several important species, which are used for food and forage. In addition, symbiosis with Rhizobium and other related bacteria for nitrogen fixation is an important characteristic in the legume root hairs (Brewin, 1991). Furthermore, Gregor Mendel used the garden pea (Pisum sativum) in breeding experiments in establishing the laws of inheritance (Mendel, 1866). To date, two of Mendel’s genes have been isolated and characterized using molecular techniques (Bhattacharya et al., 1990; Lester et al., 1997). In addition to these genetic studies, legume plants have been used in many physiological studies on environmental responses, metabolism, develop-ment, biotic interactions, and so on. Recently, two plant species, Lotus japonicus and Medicago truncatula, have emerged as model legumes, because of the advantageous features like small genome size, short generation time, and self-compatibility for whole-genome analysis (Handberg and Stougaard, 1992; Cook, 1999). Using these two legumes, several physiological phenomena have been dissected out with newer molecular technology tools (VandenBosch and Stacey, 2003).

Original languageEnglish
Title of host publicationHandbook of New Technologies for Genetic Improvement of Legumes
PublisherCRC Press
Number of pages10
ISBN (Electronic)9781439801352
ISBN (Print)9781560223085
Publication statusPublished - 2008 Jan 1


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