TY - JOUR
T1 - Gene expression profiles of cryopreserved CD34+ human umbilical cord blood cells are related to their bone marrow reconstitution abilities in mouse xenografts
AU - Sudo, Kazuhiro
AU - Yasuda, Jun
AU - Nakamura, Yukio
N1 - Funding Information:
We thank Drs. Tetsuo Noda and Yoshihide Hayashizaki for providing resources, and the DNA Chip Research Inc. (Yokohama, Japan) for its technical assistance. We obtained human UCBs from the Cell Engineering Division of RIKEN BioResource Center, which is supported by the Project for Realization of Regenerative Medicine and the National Bio-Resources Project of the MEXT.
Funding Information:
This work was supported by a grant from the Ministry of Education, Culture, Sports, Science, and Technology in Japan (MEXT) through the RIKEN Strategic Research Programs for Research and Development and was also supported by a Grant-in-aid for Scientific Research to J.Y. and by Global COE Program (Network Medicine) , MEXT. All authors declare no competing financial interests.
PY - 2010/7
Y1 - 2010/7
N2 - Human umbilical cord blood (UCB) cells are an alternative source of hematopoietic stem cells for treatment of leukemia and other diseases. It is very difficult to assess the quality of UCB cells in the clinical situation. Here, we sought to assess the quality of UCB cells by transplantation to immunodeficient mice. Cryopreserved CD34+ UCB cells from twelve different human donors were transplanted into sublethally irradiated NOD/shi-scid Jic mice. In parallel, the gene expression profiles of the UCB cells were determined from oligonucleotide microarrays. UCB cells from three donors failed to establish an engraftment in the host mice, while the other nine succeeded to various extents. Gene expression profiling indicated that 71 genes, including HOXB4, C/EBP-β, and ETS2, were specifically overexpressed and 23 genes were suppressed more than 2-fold in the successful UCB cells compared to those that failed. Functional annotation revealed that cell growth and cell cycle regulators were more abundant in the successful UCB cells. Our results suggest that hematopoietic ability may vary among cryopreserved UCB cells and that this ability can be distinguished by profiling expression of certain sets of genes.
AB - Human umbilical cord blood (UCB) cells are an alternative source of hematopoietic stem cells for treatment of leukemia and other diseases. It is very difficult to assess the quality of UCB cells in the clinical situation. Here, we sought to assess the quality of UCB cells by transplantation to immunodeficient mice. Cryopreserved CD34+ UCB cells from twelve different human donors were transplanted into sublethally irradiated NOD/shi-scid Jic mice. In parallel, the gene expression profiles of the UCB cells were determined from oligonucleotide microarrays. UCB cells from three donors failed to establish an engraftment in the host mice, while the other nine succeeded to various extents. Gene expression profiling indicated that 71 genes, including HOXB4, C/EBP-β, and ETS2, were specifically overexpressed and 23 genes were suppressed more than 2-fold in the successful UCB cells compared to those that failed. Functional annotation revealed that cell growth and cell cycle regulators were more abundant in the successful UCB cells. Our results suggest that hematopoietic ability may vary among cryopreserved UCB cells and that this ability can be distinguished by profiling expression of certain sets of genes.
KW - Gene expression profile
KW - Transplantation
KW - Umbilical cord blood
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U2 - 10.1016/j.bbrc.2010.06.010
DO - 10.1016/j.bbrc.2010.06.010
M3 - Article
C2 - 20570655
AN - SCOPUS:77954385725
SN - 0006-291X
VL - 397
SP - 697
EP - 705
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
