Generation of 7137 non-redundant expressed sequence tags from a legume, Lotus japonicus

Erika Asamizu, Yasukazu Nakamura, Shusei Sato, Satoshi Tabata

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)


For comprehensive analysis of genes expressed in a model legume, Lotus japonicus, a total of 22,983 5′ end expressed sequence tags (ESTs) were accumulated from normalized and size-selected cDNA libraries constructed from young (2 weeks old) plants. The EST sequences were clustered into 7137 non-redundant groups. Similarity search against public non-redundant protein database indicated that 3302 groups showed similarity to genes of known function, 1143 groups to hypothetical genes, and 2692 were novel sequences. Homologues of 5 nodule-specific genes which have been reported in other legume species were contained in the collected ESTs, suggesting that the EST source generated in this study will become a useful tool for identification of genes related to legume-specific biological processes.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalDNA Research
Issue number2
Publication statusPublished - 2000


  • cDNA
  • EST
  • Legume
  • Lotus japonicus


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