Generation of antibodies against prion protein by scrapie-infected cell immunization of PrP0/0 mice

Naoto Nakamura; Kazuyoshi Miyamoto; Mariko Shimokawa; Noriuki Nishida; Shirou Mohri; Tetsuyuki Kitamoto; Hiroyuki Horiuchi; Shuichi Furusawa; Haruo Matsuda

doi:10.1089/153685903322328992

Generation of antibodies against prion protein by scrapie-infected cell immunization of PrP^0/0 mice

Naoto Nakamura, Kazuyoshi Miyamoto, Mariko Shimokawa, Noriuki Nishida, Shirou Mohri, Tetsuyuki Kitamoto, Hiroyuki Horiuchi, Shuichi Furusawa, Haruo Matsuda

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Four monoclonal antibodies (MAbs) specific for prion protein (PrP) were generated by using PrP-knockout mice immunized with a scrapie-infected mouse neuroblastoma cell line (N2a/22L). The MAbs reacted with both the cellular form (PrP^C) and the protease K-treated form (PrP^Sc) on Western blotting. Of the four MAbs, three recognized mouse and hamster PrP, while the remaining MAb recognized mouse, sheep, and bovine PrPs. In addition, these MAbs were shown to react only with the unglycosylated and monoglycoslated forms of PrP^Sc in N2a/22L, but reacted with all glycosylated forms of PrP ^C and PrP^Sc from mouse brain. This study was the first to report the development of anti-PrP MAbs using scrapie-infected cells as an immunogen and provides one approach for the generation of PrP-specific MAbs.

Original language	English
Pages (from-to)	263-266
Number of pages	4
Journal	Hybridoma and Hybridomics
Volume	22
Issue number	4
DOIs	https://doi.org/10.1089/153685903322328992
Publication status	Published - 2003 Aug
Externally published	Yes

ASJC Scopus subject areas

Immunology
Genetics

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title = "Generation of antibodies against prion protein by scrapie-infected cell immunization of PrP0/0 mice",

abstract = "Four monoclonal antibodies (MAbs) specific for prion protein (PrP) were generated by using PrP-knockout mice immunized with a scrapie-infected mouse neuroblastoma cell line (N2a/22L). The MAbs reacted with both the cellular form (PrPC) and the protease K-treated form (PrPSc) on Western blotting. Of the four MAbs, three recognized mouse and hamster PrP, while the remaining MAb recognized mouse, sheep, and bovine PrPs. In addition, these MAbs were shown to react only with the unglycosylated and monoglycoslated forms of PrPSc in N2a/22L, but reacted with all glycosylated forms of PrP C and PrPSc from mouse brain. This study was the first to report the development of anti-PrP MAbs using scrapie-infected cells as an immunogen and provides one approach for the generation of PrP-specific MAbs.",

author = "Naoto Nakamura and Kazuyoshi Miyamoto and Mariko Shimokawa and Noriuki Nishida and Shirou Mohri and Tetsuyuki Kitamoto and Hiroyuki Horiuchi and Shuichi Furusawa and Haruo Matsuda",

year = "2003",

month = aug,

doi = "10.1089/153685903322328992",

language = "English",

volume = "22",

pages = "263--266",

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T1 - Generation of antibodies against prion protein by scrapie-infected cell immunization of PrP0/0 mice

AU - Nakamura, Naoto

AU - Miyamoto, Kazuyoshi

AU - Shimokawa, Mariko

AU - Nishida, Noriuki

AU - Mohri, Shirou

AU - Kitamoto, Tetsuyuki

AU - Horiuchi, Hiroyuki

AU - Furusawa, Shuichi

AU - Matsuda, Haruo

PY - 2003/8

Y1 - 2003/8

N2 - Four monoclonal antibodies (MAbs) specific for prion protein (PrP) were generated by using PrP-knockout mice immunized with a scrapie-infected mouse neuroblastoma cell line (N2a/22L). The MAbs reacted with both the cellular form (PrPC) and the protease K-treated form (PrPSc) on Western blotting. Of the four MAbs, three recognized mouse and hamster PrP, while the remaining MAb recognized mouse, sheep, and bovine PrPs. In addition, these MAbs were shown to react only with the unglycosylated and monoglycoslated forms of PrPSc in N2a/22L, but reacted with all glycosylated forms of PrP C and PrPSc from mouse brain. This study was the first to report the development of anti-PrP MAbs using scrapie-infected cells as an immunogen and provides one approach for the generation of PrP-specific MAbs.

AB - Four monoclonal antibodies (MAbs) specific for prion protein (PrP) were generated by using PrP-knockout mice immunized with a scrapie-infected mouse neuroblastoma cell line (N2a/22L). The MAbs reacted with both the cellular form (PrPC) and the protease K-treated form (PrPSc) on Western blotting. Of the four MAbs, three recognized mouse and hamster PrP, while the remaining MAb recognized mouse, sheep, and bovine PrPs. In addition, these MAbs were shown to react only with the unglycosylated and monoglycoslated forms of PrPSc in N2a/22L, but reacted with all glycosylated forms of PrP C and PrPSc from mouse brain. This study was the first to report the development of anti-PrP MAbs using scrapie-infected cells as an immunogen and provides one approach for the generation of PrP-specific MAbs.

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Generation of antibodies against prion protein by scrapie-infected cell immunization of PrP^0/0 mice

Abstract

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