TY - JOUR
T1 - Genetic analysis of DNA microsatellite loci in salivary gland tumours
T2 - Comparison with immunohistochemical detection of hMSH2 and p53 proteins
AU - Ohki, K.
AU - Kumamoto, H.
AU - Ichinohasama, R.
AU - Suzuki, M.
AU - Yamaguchi, T.
AU - Echigo, S.
AU - Motegi, K.
AU - Ooya, K.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - To investigate genetic alterations in salivary gland tumours, microsatellite instability at eight representative loci and loss of heterozygosity (LOH) on chromosome 17 were analysed by polymerase chain reaction amplification. The results were compared with immunohistochemical expression of the hMSH2 and p53 proteins. Microsatellite instability and expression loss of hMSH2 protein were not recognized in the salivary gland tumours, suggesting a low frequency of abnormalities of the mismatch repair system. LOH associated with the p53 gene was detected in approximately one-half of pleomorphic adenomas and salivary carcinomas, which often showed strong p53 immunoreactivity. These features suggest that the p53 gene plays an important role in malignant transformation of salivary gland tumours. The genetic characteristics of pleomorphic adenomas might reflect a low-grade potential for malignant progression.
AB - To investigate genetic alterations in salivary gland tumours, microsatellite instability at eight representative loci and loss of heterozygosity (LOH) on chromosome 17 were analysed by polymerase chain reaction amplification. The results were compared with immunohistochemical expression of the hMSH2 and p53 proteins. Microsatellite instability and expression loss of hMSH2 protein were not recognized in the salivary gland tumours, suggesting a low frequency of abnormalities of the mismatch repair system. LOH associated with the p53 gene was detected in approximately one-half of pleomorphic adenomas and salivary carcinomas, which often showed strong p53 immunoreactivity. These features suggest that the p53 gene plays an important role in malignant transformation of salivary gland tumours. The genetic characteristics of pleomorphic adenomas might reflect a low-grade potential for malignant progression.
KW - Immunohistochemistry
KW - Microsatellite instability
KW - Salivary gland tumour
KW - hMSH2
KW - p53
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U2 - 10.1054/ijom.2001.0161
DO - 10.1054/ijom.2001.0161
M3 - Article
C2 - 11829237
AN - SCOPUS:0035544210
SN - 0901-5027
VL - 30
SP - 538
EP - 544
JO - International Journal of Oral and Maxillofacial Surgery
JF - International Journal of Oral and Maxillofacial Surgery
IS - 6
ER -