Genetic polymorphisms of nlrp3 (Rs4612666) and card8 (rs2043211) in periodontitis and cardiovascular diseases

Jaideep Mahendra, Abirami Nayaki Rao, Little Mahendra, Hytham N. Fageeh, Hammam Ibrahim Fageeh, Thodur Madapusi Balaji, Saranya Varadarajan, Raghunathan Jagannathan, Deepak Mehta, Venkata V. Suresh, A. Thirumal Raj, Shankargouda Patil

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4 Citations (Scopus)


Background: The existing data show that inflammasomes play a role in periodontal disease pathogenesis. However, their role in the pathogenesis of periodontitis and coronary heart disease remains unclear. This study had the objective of assessing NLRP3 (rs4612666) and CARD8 (rs2043211) gene polymorphisms in dental plaque and blood of generalized chronic periodontitis (CP) patients in the presence and absence of coronary heart disease (CHD). Methods: A total of 70 subjects were divided into two groups, including CP and CP + CHD subjects. Demographic variables, periodontal, and cardiac parameters were recorded from both groups. Subgingival plaque and blood samples were obtained from both groups and were subjected to further molecular analysis for NLRP3 (rs4612666) and CARD8 (rs2043211) expression and allele change using conventional polymerase chain reaction (PCR) and gene sequencing (Sanger’s method). Results: Amongst the demographic variables, age, and monthly income were statistically significant between the two groups. Plaque index (PI), clinical attachment level (CAL), high-density lipoprotein (HDL), and low density-lipoprotein (LDL) exhibited statistically significant levels between the two groups. NLRP3 (rs4612666) and CARD8 (rs2043211) genes showed a statistically significant association of allele change (frequency) among the groups. In general, when all of the parameters were compared to the allele change of the genes, statistically significant relationships were found between the two groups. Conclusions: The present study expressed an allele change of the investigated genes which could profoundly affect the pathobiology of the two diseases under investigation.

Original languageEnglish
Article number592
Issue number7
Publication statusPublished - 2021 Jul


  • Cardiovascular disease
  • Genetic expression
  • Inflammasome
  • Periodontal medicine


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