Genome Editing of Medaka

Rie Hara, Satoshi Ansai, Masato Kinoshita

Research output: Contribution to journalArticlepeer-review


Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knockout strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knockin using a plasmid carrying a sequence that does not cause off-target effects in medaka. Additionally, knockin method without plasmid cloning is described.

Original languageEnglish
Pages (from-to)325-339
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 2023


  • CRISPR/Cas
  • Genome editing
  • Heteroduplex mobility assay
  • Medaka
  • Targeted integration
  • Targeted mutagenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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