Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite Begomovirus

Tatsuya Kon; Lolita M. Dolores; Akiko Murayama; Narceo B. Bajet; Shu Hase; Hideki Takahashi; Masato Ikegami

doi:10.1046/j.1439-0434.2002.00817.x

Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite Begomovirus

Tatsuya Kon, Lolita M. Dolores, Akiko Murayama, Narceo B. Bajet, Shu Hase, Hideki Takahashi, Masato Ikegami

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Complete nucleotide sequence of infectious cloned DNA of Tomato leaf curl virus from Philippines (ToLCV-Ph) was determined. The single circular DNA molecule comprises 2755 nucleotides. ToLCV-Ph DNA contains six open reading frames (ORFs) each capable of encoding proteins with a molecular weight greater than 10 kDa. A partial dimeric ToLCV-Ph DNA clone was constructed in a binary vector and used to agroinoculate tomato plants (Lycopersicon esculentum Mill. cv. Zuikou 102). Typical leaf curl symptoms were observed, showing that the single DNA component is sufficient for infectivity. In total nucleotide sequence comparisons with other geminiviruses, ToLCV-Ph was most closely related to Ageratum yellow vein virus (AYVV) (79% identity), ToLCV-Laos (78%), Soyabean crinkle leaf virus-Thailand (78%) and ToLCV-Taiwan (77%). The significant but relatively low sequence identity in the genomic DNA between ToLCV-Ph and other geminiviruses suggests that it is a distinct geminivirus in the genus Begomovirus.

Original language	English
Pages (from-to)	587-591
Number of pages	5
Journal	Journal of Phytopathology
Volume	150
Issue number	11-12
DOIs	https://doi.org/10.1046/j.1439-0434.2002.00817.x
Publication status	Published - 2002 Dec

Keywords

Begomovirus
Geminiviridae
Nucleotide sequence
Tomato leaf curl virus

Access to Document

10.1046/j.1439-0434.2002.00817.x

Cite this

@article{8d22383238184ea5b04953f1ac321e38,

title = "Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite Begomovirus",

abstract = "Complete nucleotide sequence of infectious cloned DNA of Tomato leaf curl virus from Philippines (ToLCV-Ph) was determined. The single circular DNA molecule comprises 2755 nucleotides. ToLCV-Ph DNA contains six open reading frames (ORFs) each capable of encoding proteins with a molecular weight greater than 10 kDa. A partial dimeric ToLCV-Ph DNA clone was constructed in a binary vector and used to agroinoculate tomato plants (Lycopersicon esculentum Mill. cv. Zuikou 102). Typical leaf curl symptoms were observed, showing that the single DNA component is sufficient for infectivity. In total nucleotide sequence comparisons with other geminiviruses, ToLCV-Ph was most closely related to Ageratum yellow vein virus (AYVV) (79% identity), ToLCV-Laos (78%), Soyabean crinkle leaf virus-Thailand (78%) and ToLCV-Taiwan (77%). The significant but relatively low sequence identity in the genomic DNA between ToLCV-Ph and other geminiviruses suggests that it is a distinct geminivirus in the genus Begomovirus.",

keywords = "Begomovirus, Geminiviridae, Nucleotide sequence, Tomato leaf curl virus",

author = "Tatsuya Kon and Dolores, {Lolita M.} and Akiko Murayama and Bajet, {Narceo B.} and Shu Hase and Hideki Takahashi and Masato Ikegami",

year = "2002",

month = dec,

doi = "10.1046/j.1439-0434.2002.00817.x",

language = "English",

volume = "150",

pages = "587--591",

journal = "Journal of Phytopathology",

issn = "0931-1785",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "11-12",

}

TY - JOUR

T1 - Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite Begomovirus

AU - Kon, Tatsuya

AU - Dolores, Lolita M.

AU - Murayama, Akiko

AU - Bajet, Narceo B.

AU - Hase, Shu

AU - Takahashi, Hideki

AU - Ikegami, Masato

PY - 2002/12

Y1 - 2002/12

N2 - Complete nucleotide sequence of infectious cloned DNA of Tomato leaf curl virus from Philippines (ToLCV-Ph) was determined. The single circular DNA molecule comprises 2755 nucleotides. ToLCV-Ph DNA contains six open reading frames (ORFs) each capable of encoding proteins with a molecular weight greater than 10 kDa. A partial dimeric ToLCV-Ph DNA clone was constructed in a binary vector and used to agroinoculate tomato plants (Lycopersicon esculentum Mill. cv. Zuikou 102). Typical leaf curl symptoms were observed, showing that the single DNA component is sufficient for infectivity. In total nucleotide sequence comparisons with other geminiviruses, ToLCV-Ph was most closely related to Ageratum yellow vein virus (AYVV) (79% identity), ToLCV-Laos (78%), Soyabean crinkle leaf virus-Thailand (78%) and ToLCV-Taiwan (77%). The significant but relatively low sequence identity in the genomic DNA between ToLCV-Ph and other geminiviruses suggests that it is a distinct geminivirus in the genus Begomovirus.

AB - Complete nucleotide sequence of infectious cloned DNA of Tomato leaf curl virus from Philippines (ToLCV-Ph) was determined. The single circular DNA molecule comprises 2755 nucleotides. ToLCV-Ph DNA contains six open reading frames (ORFs) each capable of encoding proteins with a molecular weight greater than 10 kDa. A partial dimeric ToLCV-Ph DNA clone was constructed in a binary vector and used to agroinoculate tomato plants (Lycopersicon esculentum Mill. cv. Zuikou 102). Typical leaf curl symptoms were observed, showing that the single DNA component is sufficient for infectivity. In total nucleotide sequence comparisons with other geminiviruses, ToLCV-Ph was most closely related to Ageratum yellow vein virus (AYVV) (79% identity), ToLCV-Laos (78%), Soyabean crinkle leaf virus-Thailand (78%) and ToLCV-Taiwan (77%). The significant but relatively low sequence identity in the genomic DNA between ToLCV-Ph and other geminiviruses suggests that it is a distinct geminivirus in the genus Begomovirus.

KW - Begomovirus

KW - Geminiviridae

KW - Nucleotide sequence

KW - Tomato leaf curl virus

UR - http://www.scopus.com/inward/record.url?scp=0036961255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036961255&partnerID=8YFLogxK

U2 - 10.1046/j.1439-0434.2002.00817.x

DO - 10.1046/j.1439-0434.2002.00817.x

M3 - Article

AN - SCOPUS:0036961255

SN - 0931-1785

VL - 150

SP - 587

EP - 591

JO - Journal of Phytopathology

JF - Journal of Phytopathology

IS - 11-12

ER -

Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite Begomovirus

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this