Genome-wide analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus

Aki Sagisaka; Kosuke Fujita; Yuki Nakamura; Jun Ishibashi; Hiroaki Noda; Shigeo Imanishi; Kazuei Mita; Minoru Yamakawa; Hiromitsu Tanaka

doi:10.1016/j.virusres.2009.10.015

Genome-wide analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus

Aki Sagisaka, Kosuke Fujita, Yuki Nakamura, Jun Ishibashi, Hiroaki Noda, Shigeo Imanishi, Kazuei Mita, Minoru Yamakawa, Hiromitsu Tanaka

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

The global transcriptional profile of host genes in the silkworm cell line during the early phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection was analyzed by oligonucleotide microarray. Our analysis showed 35 genes were significantly up-regulated and 17 genes were significantly down-regulated. This is the first report of changes in the expression of these genes in response to NPV infection. We further quantified the levels of mRNA expression by quantitative reverse transcriptase-polymerase chain reaction and confirmed that the expression of 13 (such as BmEts and BmToll10-3) genes significantly increased and 7 genes (such as Hsp20-1) significantly decreased after BmNPV infection. However, the expression levels of most genes were not dramatically changed except BmEts expression increased approximately 8.0-fold 12 h after BmNPV infection.

Original language	English
Pages (from-to)	166-175
Number of pages	10
Journal	Virus Research
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/j.virusres.2009.10.015
Publication status	Published - 2010 Feb
Externally published	Yes

Keywords

Bombyx mori nucleopolyhedrovirus
Gene expression
Oligonucleotide microarray
Quantitative RT-PCR
Silkworm

ASJC Scopus subject areas

Virology
Infectious Diseases
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virusres.2009.10.015

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title = "Genome-wide analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus",

abstract = "The global transcriptional profile of host genes in the silkworm cell line during the early phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection was analyzed by oligonucleotide microarray. Our analysis showed 35 genes were significantly up-regulated and 17 genes were significantly down-regulated. This is the first report of changes in the expression of these genes in response to NPV infection. We further quantified the levels of mRNA expression by quantitative reverse transcriptase-polymerase chain reaction and confirmed that the expression of 13 (such as BmEts and BmToll10-3) genes significantly increased and 7 genes (such as Hsp20-1) significantly decreased after BmNPV infection. However, the expression levels of most genes were not dramatically changed except BmEts expression increased approximately 8.0-fold 12 h after BmNPV infection.",

keywords = "Bombyx mori nucleopolyhedrovirus, Gene expression, Oligonucleotide microarray, Quantitative RT-PCR, Silkworm",

author = "Aki Sagisaka and Kosuke Fujita and Yuki Nakamura and Jun Ishibashi and Hiroaki Noda and Shigeo Imanishi and Kazuei Mita and Minoru Yamakawa and Hiromitsu Tanaka",

year = "2010",

month = feb,

doi = "10.1016/j.virusres.2009.10.015",

language = "English",

volume = "147",

pages = "166--175",

journal = "Virus Research",

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T1 - Genome-wide analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus

AU - Sagisaka, Aki

AU - Fujita, Kosuke

AU - Nakamura, Yuki

AU - Ishibashi, Jun

AU - Noda, Hiroaki

AU - Imanishi, Shigeo

AU - Mita, Kazuei

AU - Yamakawa, Minoru

AU - Tanaka, Hiromitsu

PY - 2010/2

Y1 - 2010/2

N2 - The global transcriptional profile of host genes in the silkworm cell line during the early phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection was analyzed by oligonucleotide microarray. Our analysis showed 35 genes were significantly up-regulated and 17 genes were significantly down-regulated. This is the first report of changes in the expression of these genes in response to NPV infection. We further quantified the levels of mRNA expression by quantitative reverse transcriptase-polymerase chain reaction and confirmed that the expression of 13 (such as BmEts and BmToll10-3) genes significantly increased and 7 genes (such as Hsp20-1) significantly decreased after BmNPV infection. However, the expression levels of most genes were not dramatically changed except BmEts expression increased approximately 8.0-fold 12 h after BmNPV infection.

AB - The global transcriptional profile of host genes in the silkworm cell line during the early phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection was analyzed by oligonucleotide microarray. Our analysis showed 35 genes were significantly up-regulated and 17 genes were significantly down-regulated. This is the first report of changes in the expression of these genes in response to NPV infection. We further quantified the levels of mRNA expression by quantitative reverse transcriptase-polymerase chain reaction and confirmed that the expression of 13 (such as BmEts and BmToll10-3) genes significantly increased and 7 genes (such as Hsp20-1) significantly decreased after BmNPV infection. However, the expression levels of most genes were not dramatically changed except BmEts expression increased approximately 8.0-fold 12 h after BmNPV infection.

KW - Bombyx mori nucleopolyhedrovirus

KW - Gene expression

KW - Oligonucleotide microarray

KW - Quantitative RT-PCR

KW - Silkworm

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SP - 166

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Genome-wide analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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