Genome-wide association analyses point to candidate genes for electric shock avoidance in Drosophila melanogaster

Mirjam Appel, Claus Jürgen Scholz, Tobias Müller, Marcus Dittrich, Christian König, Marie Bockstaller, Tuba Oguz, Afshin Khalili, Emmanuel Antwi-Adjei, Tamas Schauer, Carla Margulies, Hiromu Tanimoto, Ayse Yarali

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Electric shock is a common stimulus for nociception-research and the most widely used reinforcement in aversive associative learning experiments. Yet, nothing is known about the mechanisms it recruits at the periphery. To help fill this gap, we undertook a genome-wide association analysis using 38 inbred Drosophila melanogaster strains, which avoided shock to varying extents. We identified 514 genes whose expression levels and/ or sequences covaried with shock avoidance scores. We independently scrutinized 14 of these genes using mutants, validating the effect of 7 of them on shock avoidance. This emphasizes the value of our candidate gene list as a guide for follow-up research. In addition, by integrating our association results with external protein-protein interaction data we obtained a shock avoidance-associated network of 38 genes. Both this network and the original candidate list contained a substantial number of genes that affect mechanosensory bristles, which are hairlike organs distributed across the fly's body. These results may point to a potential role for mechanosensory bristles in shock sensation. Thus, we not only provide a first list of candidate genes for shock avoidance, but also point to an interesting new hypothesis on nociceptive mechanisms.

Original languageEnglish
Article numbere0126986
JournalPLoS ONE
Volume10
Issue number5
DOIs
Publication statusPublished - 2015 May 18

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