TY - JOUR
T1 - Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene
AU - Minekura, Hiroyuki
AU - Kang, Man Jong
AU - Inagaki, Yosuke
AU - Suzuki, Hiroyuki
AU - Sato, Hiroyuki
AU - Fujino, Takahiro
AU - Yamamoto, Tokuo T.
N1 - Funding Information:
We thank Dr Takafumi Kohama for helpful comments on the manuscript. This work was supported by Japan Society for Promotion of Science Grant RFTF97L00803.
PY - 2001/10/31
Y1 - 2001/10/31
N2 - Acyl-CoA synthetases (ACSs) play an essential role in fatty acid metabolism. ACS3 is an arachidonate-preferring enzyme expressed in a wide range of human tissues including brain, heart, placenta, prostate, skeletal muscle, testis and thymus. As an initial step to understanding the transcriptional regulation of the human ACS3 gene, we analyzed the genomic organization and transcription units of the human ACS3 gene. Sequence analysis of genomic clones demonstrates that the human ACS3 gene spans at least 80.6 kb and contains 17 exons. The human ACS3 gene was mapped between the sequence-tagged site markers D2S360 and WI-21901. Sequence inspection of the 5′-flanking region revealed potential DNA elements including CCAAT, AP-1, Oct-1, GATAs, SRY, CdxA, Nkx-2.5, c-Myb, HSF2, NF-AT, AP-2, NF-Y, and p300. A minimal promoter region required for the expression of the human ACS3 gene in melanoma G361 cells was determined.
AB - Acyl-CoA synthetases (ACSs) play an essential role in fatty acid metabolism. ACS3 is an arachidonate-preferring enzyme expressed in a wide range of human tissues including brain, heart, placenta, prostate, skeletal muscle, testis and thymus. As an initial step to understanding the transcriptional regulation of the human ACS3 gene, we analyzed the genomic organization and transcription units of the human ACS3 gene. Sequence analysis of genomic clones demonstrates that the human ACS3 gene spans at least 80.6 kb and contains 17 exons. The human ACS3 gene was mapped between the sequence-tagged site markers D2S360 and WI-21901. Sequence inspection of the 5′-flanking region revealed potential DNA elements including CCAAT, AP-1, Oct-1, GATAs, SRY, CdxA, Nkx-2.5, c-Myb, HSF2, NF-AT, AP-2, NF-Y, and p300. A minimal promoter region required for the expression of the human ACS3 gene in melanoma G361 cells was determined.
KW - Acyl-CoA synthetase
KW - Arachidonate
KW - Fatty acid
KW - Genomic organization
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U2 - 10.1016/S0378-1119(01)00714-4
DO - 10.1016/S0378-1119(01)00714-4
M3 - Article
C2 - 11707336
AN - SCOPUS:0035980624
SN - 0378-1119
VL - 278
SP - 185
EP - 192
JO - Gene
JF - Gene
IS - 1-2
ER -