TY - JOUR
T1 - Genomic organization, chromosomal localization, and the complete 22 kb DNA sequence of the human GCMa/GCM1, a placenta-specific transcription factor gene
AU - Yamada, Kazuyo
AU - Ogawa, Hisamitsu
AU - Tamiya, Gen
AU - Ikeno, Masashi
AU - Morita, Miwa
AU - Asakawa, Shuichi
AU - Shimizu, Nobuyoshi
AU - Okazaki, Tuneko
N1 - Funding Information:
We thank Kiyotaka Yamada for technical assistance and Dr. Sumiko Abe-Dohmae for critical reading of the manuscript. This work has been supported by a Grant-in-Aid for research from Fujita Health University and Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (11671660, 12671637).
PY - 2000/11/11
Y1 - 2000/11/11
N2 - The genomic sequence of the human GCMa/GCM1 gene, a mammalian homologue of Drosophila melanogaster GCM, was determined. Drosophila GCM is a neural transcription factor that regulates glial cell fate. The mammalian homolog however, is a placenta-specific transcription factor that is necessary for placental development. The 22 kb DNA sequence spanning the GCMa gene contains six exons and five introns, encoding a 2.8 kb cDNA. Overall genomic organization is similar for the human and mouse. Several potential binding sites for transcription factors like GATA, Oct-1, and bHLH proteins were found in the 5'-flanking region of the human gene. A DNA motif for GCM protein binding exists in the 5'-flanking region that is highly homologous with that of the mouse gene. The location of this gene was mapped to chromosome 6 using fluorescence in situ hybridization. (C) 2000 Academic Press.
AB - The genomic sequence of the human GCMa/GCM1 gene, a mammalian homologue of Drosophila melanogaster GCM, was determined. Drosophila GCM is a neural transcription factor that regulates glial cell fate. The mammalian homolog however, is a placenta-specific transcription factor that is necessary for placental development. The 22 kb DNA sequence spanning the GCMa gene contains six exons and five introns, encoding a 2.8 kb cDNA. Overall genomic organization is similar for the human and mouse. Several potential binding sites for transcription factors like GATA, Oct-1, and bHLH proteins were found in the 5'-flanking region of the human gene. A DNA motif for GCM protein binding exists in the 5'-flanking region that is highly homologous with that of the mouse gene. The location of this gene was mapped to chromosome 6 using fluorescence in situ hybridization. (C) 2000 Academic Press.
KW - GCMa/GCM1
KW - Genomic organization
KW - Placenta
KW - Transcription factor
KW - Trophoblast Specific Element
UR - http://www.scopus.com/inward/record.url?scp=0034638637&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034638637&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2000.3775
DO - 10.1006/bbrc.2000.3775
M3 - Article
C2 - 11071865
AN - SCOPUS:0034638637
SN - 0006-291X
VL - 278
SP - 134
EP - 139
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -