TY - JOUR
T1 - Genomic structure and regulation of a novel human gene, Klp1
AU - Takahashi, Shinichiro
AU - Harigae, Hideo
AU - Yokoyama, Hisayuki
AU - Kaku, Mitsuo
AU - Sasaki, Takeshi
N1 - Funding Information:
We thank Drs. K. Furuyama, S. Taketani, and Y. Ikeda for helpful comments. We also thank the members in the Department of Rheumatology and Hematology for fruitful discussions. This work is supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (H.H., T.S.).
PY - 2001/12/30
Y1 - 2001/12/30
N2 - Klp1 (K562 cells-derived leucine zipper-like protein 1) is a transcription factor which binds to the coproporphyrinogen oxidase promoter regulatory element (GGACTACAG). In order to clarify the function of Klp1, we determined the complete human Klp1 genomic structure and regulatory element in the promoter region. The gene spans about 2.4 kb and has three exons. Its promoter region has multiple GC boxes, E2F binding site, one cAMP response element (CRE), and no TATA box with multiple transcription initiation sites, which is characteristic of housekeeping and growth regulating genes. Promoter analysis showed that the promoter was more active in K562 cells entered into the cell cycle by serum stimulation than quiescent cells. Further promoter analysis revealed that CRE at -42 is essential for full promoter activity, and c-Jun and activation transcription factor 1/cAMP response element binding protein 1 proteins bind to this element. These structural characteristics and the promoter function suggest that Klp1 may play a role in cell cycle regulation.
AB - Klp1 (K562 cells-derived leucine zipper-like protein 1) is a transcription factor which binds to the coproporphyrinogen oxidase promoter regulatory element (GGACTACAG). In order to clarify the function of Klp1, we determined the complete human Klp1 genomic structure and regulatory element in the promoter region. The gene spans about 2.4 kb and has three exons. Its promoter region has multiple GC boxes, E2F binding site, one cAMP response element (CRE), and no TATA box with multiple transcription initiation sites, which is characteristic of housekeeping and growth regulating genes. Promoter analysis showed that the promoter was more active in K562 cells entered into the cell cycle by serum stimulation than quiescent cells. Further promoter analysis revealed that CRE at -42 is essential for full promoter activity, and c-Jun and activation transcription factor 1/cAMP response element binding protein 1 proteins bind to this element. These structural characteristics and the promoter function suggest that Klp1 may play a role in cell cycle regulation.
KW - Activation transcription factor/cAMP response element binding protein
KW - c-Jun
KW - cAMP response element
KW - Leucine zipper protein
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U2 - 10.1016/S0167-4781(01)00349-9
DO - 10.1016/S0167-4781(01)00349-9
M3 - Article
C2 - 11779635
AN - SCOPUS:0035977465
SN - 0167-4781
VL - 1522
SP - 207
EP - 211
JO - BBA - Gene Structure and Expression
JF - BBA - Gene Structure and Expression
IS - 3
ER -