TY - JOUR
T1 - Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst
AU - Ozawa, Manabu
AU - Sakatani, Miki
AU - Yao, Jiqiang
AU - Shanker, Savita
AU - Yu, Fahong
AU - Yamashita, Rui
AU - Wakabayashi, Shunichi
AU - Nakai, Kenta
AU - Dobbs, Kyle B.
AU - Sudano, Mateus José
AU - Farmerie, William G.
AU - Hansen, Peter J.
N1 - Funding Information:
This work was supported by Agriculture and Food Research Initiative Grant Nos. 2009-65203-05732 and 2011-67015-30688 from United States Dept. of Agriculture National Institute of Food and Agriculture and by a grant from the Research Opportunity Fund, University of Florida Research Foundation. The authors thank William Rembert, for collecting oocytes, the Chernin family and Central Packing (Center Hill, Florida), for donating ovarian tissue and Scott Randell of Southeastern Semen (Wellborn Florida), for donating semen.
PY - 2012
Y1 - 2012
N2 - Background: The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system. Results: A total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM (for example, NANOG, SOX2, and STAT3) and TE (ELF5, GATA3, and KRT18) in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human. Conclusion: Analysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast.
AB - Background: The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system. Results: A total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM (for example, NANOG, SOX2, and STAT3) and TE (ELF5, GATA3, and KRT18) in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human. Conclusion: Analysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast.
KW - Blastocyst
KW - Development
KW - Inner cell mass
KW - Trophectoderm
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U2 - 10.1186/1471-213X-12-33
DO - 10.1186/1471-213X-12-33
M3 - Article
C2 - 23126590
AN - SCOPUS:84868274013
SN - 1471-213X
VL - 12
JO - BMC Developmental Biology
JF - BMC Developmental Biology
M1 - 33
ER -