Heat inactivation of oxygen evolution in Photosystem II particles and its acceleration by chloride depletion and exogenous manganese

Diane Nash; Mitsue Miyao; Norio Murata

doi:10.1016/0005-2728(85)90115-X

Heat inactivation of oxygen evolution in Photosystem II particles and its acceleration by chloride depletion and exogenous manganese

Diane Nash, Mitsue Miyao, Norio Murata

Research output: Contribution to journal › Article › peer-review

216 Citations (Scopus)

Abstract

Heat inactivation of oxygen evolution by isolated Photosystem II particles was accelerated by Cl^- depletion and exogenous Mn²⁺. Weak red light also accelerated heat inactivation. Heat treatment released the 33, 24 and 18 kDa proteins and Mn from the Photosystem II particles. The protein release was stimulated by Cl^- depletion and exogenous Mn²⁺, and the Mn release was also stimulated by Cl^- depletion. A 50% loss of Mn corresponded to full inactivation of oxygen evolution, whereas no direct correlation seemed to exist between the loss of any one protein and inactivation of oxygen evolution. Removal of the 24 and 18 kDa proteins from photosystem II particles only slightly decreased the heat stability of oxygen evolution.

Original language	English
Pages (from-to)	127-133
Number of pages	7
Journal	BBA - Bioenergetics
Volume	807
Issue number	2
DOIs	https://doi.org/10.1016/0005-2728(85)90115-X
Publication status	Published - 1985 May 3
Externally published	Yes

Keywords

(Spinach chloroplast)
Chloride effect
Heat inactivation
Mn effect
Oxygen evolution
Photosystem II

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(85)90115-X

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title = "Heat inactivation of oxygen evolution in Photosystem II particles and its acceleration by chloride depletion and exogenous manganese",

abstract = "Heat inactivation of oxygen evolution by isolated Photosystem II particles was accelerated by Cl- depletion and exogenous Mn2+. Weak red light also accelerated heat inactivation. Heat treatment released the 33, 24 and 18 kDa proteins and Mn from the Photosystem II particles. The protein release was stimulated by Cl- depletion and exogenous Mn2+, and the Mn release was also stimulated by Cl- depletion. A 50% loss of Mn corresponded to full inactivation of oxygen evolution, whereas no direct correlation seemed to exist between the loss of any one protein and inactivation of oxygen evolution. Removal of the 24 and 18 kDa proteins from photosystem II particles only slightly decreased the heat stability of oxygen evolution.",

keywords = "(Spinach chloroplast), Chloride effect, Heat inactivation, Mn effect, Oxygen evolution, Photosystem II",

author = "Diane Nash and Mitsue Miyao and Norio Murata",

note = "Funding Information: D.N. was supported by an Australian Government Fellowship under the Australia-Japan So-ence and Technology Agreement. This work was also supported in part by Grants-in-Aid for Energy Research (59040054) and Cooperative Research (58340037) to N.M. from the Japanese Ministry of Education, Science and Culture.",

year = "1985",

month = may,

day = "3",

doi = "10.1016/0005-2728(85)90115-X",

language = "English",

volume = "807",

pages = "127--133",

journal = "Biochimica et Biophysica Acta - Bioenergetics",

issn = "0005-2728",

publisher = "Elsevier",

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TY - JOUR

T1 - Heat inactivation of oxygen evolution in Photosystem II particles and its acceleration by chloride depletion and exogenous manganese

AU - Nash, Diane

AU - Miyao, Mitsue

AU - Murata, Norio

N1 - Funding Information: D.N. was supported by an Australian Government Fellowship under the Australia-Japan So-ence and Technology Agreement. This work was also supported in part by Grants-in-Aid for Energy Research (59040054) and Cooperative Research (58340037) to N.M. from the Japanese Ministry of Education, Science and Culture.

PY - 1985/5/3

Y1 - 1985/5/3

N2 - Heat inactivation of oxygen evolution by isolated Photosystem II particles was accelerated by Cl- depletion and exogenous Mn2+. Weak red light also accelerated heat inactivation. Heat treatment released the 33, 24 and 18 kDa proteins and Mn from the Photosystem II particles. The protein release was stimulated by Cl- depletion and exogenous Mn2+, and the Mn release was also stimulated by Cl- depletion. A 50% loss of Mn corresponded to full inactivation of oxygen evolution, whereas no direct correlation seemed to exist between the loss of any one protein and inactivation of oxygen evolution. Removal of the 24 and 18 kDa proteins from photosystem II particles only slightly decreased the heat stability of oxygen evolution.

AB - Heat inactivation of oxygen evolution by isolated Photosystem II particles was accelerated by Cl- depletion and exogenous Mn2+. Weak red light also accelerated heat inactivation. Heat treatment released the 33, 24 and 18 kDa proteins and Mn from the Photosystem II particles. The protein release was stimulated by Cl- depletion and exogenous Mn2+, and the Mn release was also stimulated by Cl- depletion. A 50% loss of Mn corresponded to full inactivation of oxygen evolution, whereas no direct correlation seemed to exist between the loss of any one protein and inactivation of oxygen evolution. Removal of the 24 and 18 kDa proteins from photosystem II particles only slightly decreased the heat stability of oxygen evolution.

KW - (Spinach chloroplast)

KW - Chloride effect

KW - Heat inactivation

KW - Mn effect

KW - Oxygen evolution

KW - Photosystem II

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JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

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ER -

Heat inactivation of oxygen evolution in Photosystem II particles and its acceleration by chloride depletion and exogenous manganese

Abstract

Keywords

ASJC Scopus subject areas

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