TY - JOUR
T1 - Hemagglutination mediated by the spike protein of cell-adapted bovine torovirus
AU - Shimabukuro, Kozue
AU - Ujike, Makoto
AU - Ito, Toshihiro
AU - Tsunemitsu, Hiroshi
AU - Oshitani, Hitoshi
AU - Taguchi, Fumihiro
N1 - Funding Information:
We thank Yasushi Ami and Asuka Hirai at the National Institute of Infectious Diseases, and Hiroshi Aoki at NVLU for discussions. This work was financially supported in part by the MEXT-Supported Program for the Strategic Research Foundation at Private Universities, 2008–2012, from the Ministry of Education, Culture, Sports, Science and Technology.
PY - 2013/7
Y1 - 2013/7
N2 - Bovine torovirus (BToV)-Aichi, recently isolated in cultured cells, showed hemagglutination (HA) activity, although the virus has a truncated hemagglutinin-esterase (HE) protein, judging from its gene structure, indicating the existence of another viral protein with HA activity. We examined whether the spike (S) protein possesses HA activity. A BToV antiserum used in this study, reactive to S but not to HE, inhibited HA activity. Furthermore, cells infected with BToV and those expressing S showed hemadsorption (HAD) activity, which was inhibited by the anti-BToV serum; however, HAD activity by expressed HE was not blocked. These data indicate that the S protein of BToV-Aichi is responsible for its HA activity.
AB - Bovine torovirus (BToV)-Aichi, recently isolated in cultured cells, showed hemagglutination (HA) activity, although the virus has a truncated hemagglutinin-esterase (HE) protein, judging from its gene structure, indicating the existence of another viral protein with HA activity. We examined whether the spike (S) protein possesses HA activity. A BToV antiserum used in this study, reactive to S but not to HE, inhibited HA activity. Furthermore, cells infected with BToV and those expressing S showed hemadsorption (HAD) activity, which was inhibited by the anti-BToV serum; however, HAD activity by expressed HE was not blocked. These data indicate that the S protein of BToV-Aichi is responsible for its HA activity.
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U2 - 10.1007/s00705-013-1636-4
DO - 10.1007/s00705-013-1636-4
M3 - Article
C2 - 23420207
AN - SCOPUS:84879685842
SN - 0304-8608
VL - 158
SP - 1561
EP - 1566
JO - Archives of Virology
JF - Archives of Virology
IS - 7
ER -