TY - JOUR
T1 - Heterotrimeric G protein signaling governs the cortical stability during apical constriction in Drosophila gastrulation
AU - Kanesaki, Takuma
AU - Hirose, Susumu
AU - Grosshans, Joerg
AU - Fuse, Naoyuki
N1 - Funding Information:
We thank Drs. F. Yu, F. Matsuzaki, S. Hayashi, H. Oda, Y. Hiromi, and E. Wieschaus for providing materials, Dr. Y. Hiromi and colleagues of NIG for suggestions and discussions, and Dr. E. Nakajima for critical reading of the manuscript. We also thank Kyoto Drosophila Genetics Resource Center, Bloomington Drosophila Stock Center, The Developmental Studies Hybridoma Bank, and The Drosophila Genomics Resource Center for providing us fly strains, antibodies and DNA constructs. This work was supported by a Grant-in-Aid for Science Research from the Ministry of Education, Science, Culture and Sports of Japan.
PY - 2013/2
Y1 - 2013/2
N2 - During gastrulation in Drosophila melanogaster, coordinated apical constriction of the cellular surface drives invagination of the mesoderm anlage. Forces generated by the cortical cytoskeletal network have a pivotal role in this cellular shape change. Here, we show that the organisation of cortical actin is essential for stabilisation of the cellular surface against contraction. We found that mutation of genes related to heterotrimeric G protein (HGP) signaling, such as Gβ13F, Gγ1, and ric-8, results in formation of blebs on the ventral cellular surface. The formation of blebs is caused by perturbation of cortical actin and induced by local surface contraction. HGP signaling mediated by two Gα subunits, Concertina and G-iα65A, constitutively regulates actin organisation. We propose that the organisation of cortical actin by HGP is required to reinforce the cortex so that the cells can endure hydrostatic stress during tissue folding.
AB - During gastrulation in Drosophila melanogaster, coordinated apical constriction of the cellular surface drives invagination of the mesoderm anlage. Forces generated by the cortical cytoskeletal network have a pivotal role in this cellular shape change. Here, we show that the organisation of cortical actin is essential for stabilisation of the cellular surface against contraction. We found that mutation of genes related to heterotrimeric G protein (HGP) signaling, such as Gβ13F, Gγ1, and ric-8, results in formation of blebs on the ventral cellular surface. The formation of blebs is caused by perturbation of cortical actin and induced by local surface contraction. HGP signaling mediated by two Gα subunits, Concertina and G-iα65A, constitutively regulates actin organisation. We propose that the organisation of cortical actin by HGP is required to reinforce the cortex so that the cells can endure hydrostatic stress during tissue folding.
KW - Bleb
KW - Cortical actin
KW - Drosophila
KW - Gastrulation
KW - Heterotrimeric G protein
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U2 - 10.1016/j.mod.2012.10.001
DO - 10.1016/j.mod.2012.10.001
M3 - Article
C2 - 23085574
AN - SCOPUS:84873522426
SN - 2667-291X
VL - 130
SP - 132
EP - 142
JO - Cells and Development
JF - Cells and Development
IS - 2-3
ER -