TY - JOUR
T1 - High-performance liquid chromatographic separation of bile acid n-acetylglucosaminides
AU - Niwa, Toshifumi
AU - Koshiyama, Tomoyoshi
AU - Goto, Junichi
AU - Nambara, Toshio
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The separation of 3-N-acetylglucosaminides of cholate, chenodeoxycholate, deoxycholate, ursodeoxycholate and lithocholate, and their glycine- and taurine-conjugates has been carried out by high-performance liquid chromatography on a reversed-phase column. The chromatographic behavior of bile acid 3-N-acetylglucosaminides was dependent on the number and positions of hydroxyl groups and the structure of the side-chain. The bile acid 3-N-acetylglucosaminides were efficiently separated according to their conjugated form on a Cosmosil 5C18column using a 0.3% potassium phosphate buffer-aceto-nitrile system. The chromatographic separation of ursodeoxycholate 3- and 7-N-acetylglucosaminides is also discussed.
AB - The separation of 3-N-acetylglucosaminides of cholate, chenodeoxycholate, deoxycholate, ursodeoxycholate and lithocholate, and their glycine- and taurine-conjugates has been carried out by high-performance liquid chromatography on a reversed-phase column. The chromatographic behavior of bile acid 3-N-acetylglucosaminides was dependent on the number and positions of hydroxyl groups and the structure of the side-chain. The bile acid 3-N-acetylglucosaminides were efficiently separated according to their conjugated form on a Cosmosil 5C18column using a 0.3% potassium phosphate buffer-aceto-nitrile system. The chromatographic separation of ursodeoxycholate 3- and 7-N-acetylglucosaminides is also discussed.
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U2 - 10.1080/10826079308020916
DO - 10.1080/10826079308020916
M3 - Article
AN - SCOPUS:0027471825
SN - 1082-6076
VL - 16
SP - 331
EP - 341
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 2
ER -