High plasma osteopontin level and its relationship with interleukin-12-mediated type 1 T helper cell response in tuberculosis

Yoshinobu Koguchi, Kazuyoshi Kawakami, Kaori Uezu, Kiyoyasu Fukushima, Sigeyuki Kon, Masahiro Maeda, Atsushi Nakamoto, Isoko Owan, Mutsuo Kuba, Norifumi Kudeken, Masato Azuma, Satomi Yara, Takashi Shinzato, Futoshi Higa, Masao Tateyama, Jun Ichi Kadota, Hiroshi Mukae, Shigeru Kohno, Toshimitsu Uede, Atsushi Saito

Research output: Contribution to journalArticlepeer-review

87 Citations (Scopus)


Osteopontin (OPN, also known as Eta-1), a noncollagenous matrix protein produced by macrophages and T lymphocytes, is expressed in granulomatous lesions caused by Mycobacterium tuberculosis infection. In the present study, we compared plasma concentrations of OPN in patients with active pulmonary tuberculosis with those of healthy control subjects and patients with sarcoidosis, another disease associated with granuloma formation. Plasma OPN levels were significantly higher in patients with tuberculosis (n = 48) than in control subjects (n = 34) and patients with sarcoidosis (n = 20). OPN levels correlated well with severity of pulmonary tuberculosis, as indicated by the size of lung lesions on chest X-ray films. Furthermore, chemotherapy resulted in a significant fall in plasma OPN levels. In patients with tuberculosis, plasma OPN concentrations correlated significantly with those of interleukin (IL)-12. In vitro experiments showed that OPN production by peripheral blood mononuclear cells infected with Mycobacterium bovis bacillus Calmette-Guérin preceded the synthesis of IL-12 and interferon-γ and that the neutralizing anti-OPN monoclonal antibody significantly reduced the production of IL-12 and interferon-γ. Our results suggest that OPN may be involved in the pathologic process associated with active pulmonary tuberculosis by inducing IL-12-mediated type 1 T helper cell responses.

Original languageEnglish
Pages (from-to)1355-1359
Number of pages5
JournalAmerican journal of respiratory and critical care medicine
Issue number10
Publication statusPublished - 2003 May 15
Externally publishedYes


  • Interleukin-12
  • Mycobacterium boris bacillus Calmette-Guérin
  • Osteopontin
  • Sarcoidosis
  • Tuberculosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine


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