HIGH RESOLUTION, ONE-SHOT IMAGING OF CELLULAR ACTIVITY OF SPHEROIDS BY OXYGEN DEPENDENT ELECTROCHEMILUMINESCENCE

Kaoru Hiramoto, Kosuke Ino, Keika Komatsu, Yuji Nashimoto, Hitoshi Shiku

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We propose a facile imaging technique to visualize O2 consumption of cellular spheroids using electrochemiluminescence (ECL) of L-012, a luminol analog. The ECL was generated via electrochemical reduction of O2, so that the luminescence intensity directly corresponded to the concentration of O2, which further utilized for visualizing O2 consumption profile of spheroids. The proposed system offers high throughput imaging of cellular activity of multiple samples with comparable resolution to a conventional electrochemical method.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1443-1444
Number of pages2
ISBN (Electronic)9781733419031
Publication statusPublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 2021 Oct 102021 Oct 14

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period21/10/1021/10/14

Keywords

  • Electrochemiluminescence
  • Live cell imaging
  • Oxygen consumption
  • Spheroid

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