@article{f39546fe99144e1eb45533dd29446dcc,
title = "High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates",
abstract = "Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases. Seiriki et al. developed a very high-speed serial-sectioning imaging system named FAST that allows whole-brain imaging at a spatial resolution to image all brain cells and long-range neuronal projections in experimental animal models and facilitates animal-to-human translational research.",
keywords = "cell distribution, human post-mortem brain, non-human primate brain, subcellular resolution, whole-brain imaging",
author = "Kaoru Seiriki and Atsushi Kasai and Takeshi Hashimoto and Wiebke Schulze and Misaki Niu and Shun Yamaguchi and Takanobu Nakazawa and Inoue, {Ken ichi} and Shiori Uezono and Masahiko Takada and Yuichiro Naka and Hisato Igarashi and Masato Tanuma and Waschek, {James A.} and Yukio Ago and Tanaka, {Kenji F.} and Atsuko Hayata-Takano and Kazuki Nagayasu and Norihito Shintani and Ryota Hashimoto and Yasuto Kunii and Mizuki Hino and Junya Matsumoto and Hirooki Yabe and Takeharu Nagai and Katsumasa Fujita and Toshio Matsuda and Kazuhiro Takuma and Akemichi Baba and Hitoshi Hashimoto",
note = "Funding Information: This work was supported in part by JSPS KAKENHI grant numbers JP26293020 (H.H.), JP26670122 (H.H.), JP15H01288 (H.H.), JP17H03989 (H.H.), JP15K14964 (A.K.), JP17H05054 (A.K.), JP15H01431 (K.I.), JP16H02454 (M. Takada), JP15K19739 (Y.K.), JP26251018 (T. Nagai); the JSPS Funding Program for Next Generation World-Leading Researchers , grant number LS081 (H.H.); the JSPS Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers , grant number S2603 (H.H.); the JSPS Research Fellowships for Young Scientists , grant number JP14J01466 (K.S.); MEXT KAKENHI grant numbers JP23115003 and JP23115001 (T. Nagai); the SRPBS and Brain/MINDS from AMED (H.H.); and grants from the Takeda Science Foundation , Japan (A.K.) and the Uehara Memorial Foundation , Japan (H.H.). Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",
year = "2017",
month = jun,
day = "21",
doi = "10.1016/j.neuron.2017.05.017",
language = "English",
volume = "94",
pages = "1085--1100.e6",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",
}
