High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates

Kaoru Seiriki, Atsushi Kasai, Takeshi Hashimoto, Wiebke Schulze, Misaki Niu, Shun Yamaguchi, Takanobu Nakazawa, Ken ichi Inoue, Shiori Uezono, Masahiko Takada, Yuichiro Naka, Hisato Igarashi, Masato Tanuma, James A. Waschek, Yukio Ago, Kenji F. Tanaka, Atsuko Hayata-Takano, Kazuki Nagayasu, Norihito Shintani, Ryota HashimotoYasuto Kunii, Mizuki Hino, Junya Matsumoto, Hirooki Yabe, Takeharu Nagai, Katsumasa Fujita, Toshio Matsuda, Kazuhiro Takuma, Akemichi Baba, Hitoshi Hashimoto

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)


Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases. Seiriki et al. developed a very high-speed serial-sectioning imaging system named FAST that allows whole-brain imaging at a spatial resolution to image all brain cells and long-range neuronal projections in experimental animal models and facilitates animal-to-human translational research.

Original languageEnglish
Pages (from-to)1085-1100.e6
Issue number6
Publication statusPublished - 2017 Jun 21


  • cell distribution
  • human post-mortem brain
  • non-human primate brain
  • subcellular resolution
  • whole-brain imaging


Dive into the research topics of 'High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates'. Together they form a unique fingerprint.

Cite this