High-speed autofocusing of a cell using diffraction patterns

Hiromasa Oku, Masatoshi Ishikawa, Theodorus, Koichi Hashimoto

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


This paper proposes a new autofocusing method for observing cells under a transmission illumination. The focusing method uses a quick and simple focus estimation technique termed "depth from diffraction," which is based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the method, it was applied to continuous focus tracking of a swimming paramecium, in combination with two-dimensional position tracking. Three-dimensional tracking of the Paramecium for 70 s was successfully demonstrated.

Original languageEnglish
Pages (from-to)3952-3960
Number of pages9
JournalOptics Express
Issue number9
Publication statusPublished - 2006


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