TY - GEN
T1 - High-throughput fluorescence polarization measurement system towards molecular interaction analysis
AU - Wakao, Osamu
AU - Sumiyoshi, Ken
AU - Mizokuchi, Chikaaki
AU - Maeki, Masatoshi
AU - Ishida, Akihiko
AU - Tani, Hirofumi
AU - Shigemura, Koji
AU - Hibara, Akihide
AU - Tokeshi, Manabu
N1 - Funding Information:
A part of this work was supported by the JST-SENTAN program. O.W. acknowledges the Grant-in-Aid for JSPS Fellows 16J01317.
Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - This report demonstrated simultaneous fluorescence polarization (FP) measurement for 100 samples. The measurement was based on the synchronization detection between the switching of a liquid crystal (LC) layer and the sampling rate of an image sensor. This offered captured FP signals from multisample as single FP image, and the values of FP signals were obtained separately by image analysis. The results showed the analytical performance of our system was comparable to that of conventional apparatus and which implies our system can be applicable to molecular interaction analysis.
AB - This report demonstrated simultaneous fluorescence polarization (FP) measurement for 100 samples. The measurement was based on the synchronization detection between the switching of a liquid crystal (LC) layer and the sampling rate of an image sensor. This offered captured FP signals from multisample as single FP image, and the values of FP signals were obtained separately by image analysis. The results showed the analytical performance of our system was comparable to that of conventional apparatus and which implies our system can be applicable to molecular interaction analysis.
KW - FPIA
KW - Fluorescence Polarization
KW - Molecular Interaction Analysis
UR - http://www.scopus.com/inward/record.url?scp=85079621585&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079621585&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079621585
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 557
EP - 558
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -