TY - JOUR
T1 - High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure
AU - Shimazu, Yoshiaki
AU - Tono, Kensuke
AU - Tanaka, Tomoyuki
AU - Yamanaka, Yasuaki
AU - Nakane, Takanori
AU - Mori, Chihiro
AU - Kimura, Kanako Terakado
AU - Fujiwara, Takaaki
AU - Sugahara, Michihiro
AU - Tanaka, Rie
AU - Doak, R. Bruce
AU - Shimamura, Tatsuro
AU - Iwata, So
AU - Nango, Eriko
AU - Yabashi, Makina
N1 - Funding Information:
XFEL experiments were conducted at BL3 of SACLA with the approval of the Japan Synchrotron Radiation Research Institute (JASRI) (proposal Nos. 2014A8032, 2014B8050, 2015A8048, 2015B8042, 2016A8041, 2016B8060). SI, TS and KT acknowledge financial support from the X-ray Free-Electron Laser Priority Strategy Program from the Ministry of Education, Culture, Sports, Science and Technology (MEXT). SI acknowledges the Platform Project for Supporting Drug Discovery and Life Science Research [Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)] from Japan Agency for Medical Research and Development (AMED).
Publisher Copyright:
© 2019 BMJ Publishing Group. All rights reserved.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - A sample-injection device has been developed at SPring-8 Angstrom Compact Free-Electron Laser (SACLA) for serial femtosecond crystallography (SFX) at atmospheric pressure. Microcrystals embedded in a highly viscous carrier are stably delivered from a capillary nozzle with the aid of a coaxial gas flow and a suction device. The cartridge-type sample reservoir is easily replaceable and facilitates sample reloading or exchange. The reservoir is positioned in a cooling jacket with a temperature-regulated water flow, which is useful to prevent drastic changes in the sample temperature during data collection. This work demonstrates that the injector successfully worked in SFX of the human A2A adenosine receptor complexed with an antagonist, ZM241385, in lipidic cubic phase and for hen egg-white lysozyme microcrystals in a grease carrier. The injection device has also been applied to many kinds of proteins, not only for static structural analyses but also for dynamics studies using pump-probe techniques.
AB - A sample-injection device has been developed at SPring-8 Angstrom Compact Free-Electron Laser (SACLA) for serial femtosecond crystallography (SFX) at atmospheric pressure. Microcrystals embedded in a highly viscous carrier are stably delivered from a capillary nozzle with the aid of a coaxial gas flow and a suction device. The cartridge-type sample reservoir is easily replaceable and facilitates sample reloading or exchange. The reservoir is positioned in a cooling jacket with a temperature-regulated water flow, which is useful to prevent drastic changes in the sample temperature during data collection. This work demonstrates that the injector successfully worked in SFX of the human A2A adenosine receptor complexed with an antagonist, ZM241385, in lipidic cubic phase and for hen egg-white lysozyme microcrystals in a grease carrier. The injection device has also been applied to many kinds of proteins, not only for static structural analyses but also for dynamics studies using pump-probe techniques.
KW - microcrystal injection
KW - room-temperature crystallography
KW - serial femtosecond crystallography
KW - X-ray free-electron lasers
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U2 - 10.1107/S1600576719012846
DO - 10.1107/S1600576719012846
M3 - Article
AN - SCOPUS:85075797541
SN - 0021-8898
VL - 52
SP - 1280
EP - 1288
JO - Journal of Applied Crystallography
JF - Journal of Applied Crystallography
ER -