TY - JOUR
T1 - Highly efficient and minimally invasive transfection using time-controlled irradiation of atmospheric-pressure plasma
AU - Sasaki, Shota
AU - Kanzaki, Makoto
AU - Kaneko, Toshiro
PY - 2014/2
Y1 - 2014/2
N2 - To develop a highly efficient and minimally invasive gene transfection method, the cells after direct plasma irradiation under various conditions are evaluated by simultaneous analysis of transfection efficiency and cell viability. As a result, the efficiency has a maximal value at a short plasma irradiation time (3-5 s) while maintaining a very high cell viability, and the volume of irradiated cell suspension changes the time dependence of the efficiency, which could be caused by the competition between the synergetic effects of reactive oxygen species and electric field stimulation, and membrane transport such as exocytosis which is the process of excretion.
AB - To develop a highly efficient and minimally invasive gene transfection method, the cells after direct plasma irradiation under various conditions are evaluated by simultaneous analysis of transfection efficiency and cell viability. As a result, the efficiency has a maximal value at a short plasma irradiation time (3-5 s) while maintaining a very high cell viability, and the volume of irradiated cell suspension changes the time dependence of the efficiency, which could be caused by the competition between the synergetic effects of reactive oxygen species and electric field stimulation, and membrane transport such as exocytosis which is the process of excretion.
UR - http://www.scopus.com/inward/record.url?scp=84893266001&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893266001&partnerID=8YFLogxK
U2 - 10.7567/APEX.7.026202
DO - 10.7567/APEX.7.026202
M3 - Article
AN - SCOPUS:84893266001
SN - 1882-0778
VL - 7
JO - Applied Physics Express
JF - Applied Physics Express
IS - 2
M1 - 026202
ER -