@article{0305ac649b3941fb9d2b1ddea8328510,
title = "Hippocampal LTP is accompanied by enhanced F-actin content within the dendritic spine that is essential for late LTP maintenance in vivo",
abstract = "The dendritic spine is an important site of neuronal plasticity and contains extremely high levels of cytoskeletal actin. However, the dynamics of the actin cytoskeleton during synaptic plasticity and its in vivo function remain unclear. Here we used an in vivo dentate gyrus LTP model to show that LTP induction is associated with actin cytoskeletal reorganization characterized by a long-lasting increase in F-actin content within dendritic spines. This increase in F-actin content is dependent on NMDA receptor activation and involves the inactivation of actin depolymerizing factor/cofilin. Inhibition of actin polymerization with latrunculin A impaired late phase of LTP without affecting the initial amplitude and early maintenance of LTP. These observations suggest that mechanisms regulating the spine actin cytoskeleton contribute to the persistence of LTP.",
author = "Yugo Fukazawa and Yoshito Saitoh and Fumiko Ozawa and Yasuhiko Ohta and Kensaku Mizuno and Kaoru Inokuchi",
note = "Funding Information: We thank Drs. T. Obinata (Chiba University) and Y. Arimatsu (MITILS) for the monoclonal mouse anti-cofilin antibody and the rabbit anti-neuron-specific β-tubulin antibody, respectively, and K. Ohashi, M. Takahashi, H. Ohnishi, and S. Ono for their helpful discussions. We also thank A. Murayama for technical assistance. This work was performed through Special Coordinate Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government. This work was supported in part by Grant-in-Aid for Scientific Research (no. 14780610) to Y.F. and by grants for Scientific Research on Priority Areas (A)-Neural Circuit Project and (C)-Advanced Brain Science Project to K.I. from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government.",
year = "2003",
month = may,
day = "8",
doi = "10.1016/S0896-6273(03)00206-X",
language = "English",
volume = "38",
pages = "447--460",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",
}