TY - JOUR
T1 - Human arylhydrocarbon receptor
T2 - Functional expression and chromosomal assignment to 7p21
AU - Ema, Masatsugu
AU - Matsushita, Natsuki
AU - Sogawa, Kazuhiro
AU - Ariyama, Takeshi
AU - Inazawa, Johji
AU - Nemoto, Takayuki
AU - Ota, Minoru
AU - Oshimura, Mitsuo
AU - Fujii-kuriyama, Yoshiaki
PY - 1994/10
Y1 - 1994/10
N2 - We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. The overall amino acid identity with mouse AhR from cell line Hepa-1 is 72.5%. The human AhR expressed either in COS-7 cells or in a reticulocyte lysate in vitro translation system showed specific dioxin-binding activity and Arnt-dependent DNA-binding activity. Chromosomal localization of the AhR gene was determined to be chromosome 7p21 by fluorescent in situ hybridization and DNA blot hybridization using 23 human×mouse or Chinese hamster hybrid cell DNAs.
AB - We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. The overall amino acid identity with mouse AhR from cell line Hepa-1 is 72.5%. The human AhR expressed either in COS-7 cells or in a reticulocyte lysate in vitro translation system showed specific dioxin-binding activity and Arnt-dependent DNA-binding activity. Chromosomal localization of the AhR gene was determined to be chromosome 7p21 by fluorescent in situ hybridization and DNA blot hybridization using 23 human×mouse or Chinese hamster hybrid cell DNAs.
KW - Ah receptor
KW - Chromosomal localization
KW - Dioxin binding activity
KW - XRE binding activity
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U2 - 10.1093/oxfordjournals.jbchem.a124605
DO - 10.1093/oxfordjournals.jbchem.a124605
M3 - Article
C2 - 7883760
AN - SCOPUS:0028149537
SN - 0021-924X
VL - 116
SP - 845
EP - 851
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 4
ER -