Human arylhydrocarbon receptor: Functional expression and chromosomal assignment to 7p21

Masatsugu Ema, Natsuki Matsushita, Kazuhiro Sogawa, Takeshi Ariyama, Johji Inazawa, Takayuki Nemoto, Minoru Ota, Mitsuo Oshimura, Yoshiaki Fujii-kuriyama

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)


    We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. The overall amino acid identity with mouse AhR from cell line Hepa-1 is 72.5%. The human AhR expressed either in COS-7 cells or in a reticulocyte lysate in vitro translation system showed specific dioxin-binding activity and Arnt-dependent DNA-binding activity. Chromosomal localization of the AhR gene was determined to be chromosome 7p21 by fluorescent in situ hybridization and DNA blot hybridization using 23 human×mouse or Chinese hamster hybrid cell DNAs.

    Original languageEnglish
    Pages (from-to)845-851
    Number of pages7
    JournalJournal of biochemistry
    Issue number4
    Publication statusPublished - 1994 Oct


    • Ah receptor
    • Chromosomal localization
    • Dioxin binding activity
    • XRE binding activity

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology


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