Human BAC contig covering the deleted region in pancreatic cancer at 12q21

Emile M. Youssef; Kenzo Kaneko; Toshimasa Yatsuoka; Yutaka Hayashi; Masato Hoshi; Akira Horii; Toru Furukawa

doi:10.3109/10425170109041339

Human BAC contig covering the deleted region in pancreatic cancer at 12q21

Emile M. Youssef, Kenzo Kaneko, Toshimasa Yatsuoka, Yutaka Hayashi, Masato Hoshi, Akira Horii, Toru Furukawa

MED - Medical Sciences

Tohoku University

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

In sporadic human primary pancreatic cancer tissues, loss of heterozygosity is frequently observed in the 1-cM region between D12S81 and D12S1719 at 12q21. Loss of this chromosome arm is known to be associate with a poor prognosis in pancreatic cancer patients. Herein we report a complete contig of human bacterial artificial chromosome (BAC) clones covering the deleted region. The region was covered by 21 BAC clones in a minimum tiling path. The clones were confirmed to exist at 12q21 by fluorescence In situ hybridization. We identified novel 40 sequence tagged sites and mapped 10 expressed sequence tags in this region. The BAC contig reported here provides an avenue for determining the complete nucleotide sequence and mining putative tumor suppressor genes in the deleted region of pancreatic cancer at 12q21.

Original language	English
Pages (from-to)	541-546
Number of pages	6
Journal	Mitochondrial DNA
Volume	11
Issue number	6
DOIs	https://doi.org/10.3109/10425170109041339
Publication status	Published - 2001

Keywords

12q21
bacterial artificial chromosome
expressed sequence tag
pancreatic cancer
physical map
sequence tagged site

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/10425170109041339

Cite this

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title = "Human BAC contig covering the deleted region in pancreatic cancer at 12q21",

abstract = "In sporadic human primary pancreatic cancer tissues, loss of heterozygosity is frequently observed in the 1-cM region between D12S81 and D12S1719 at 12q21. Loss of this chromosome arm is known to be associate with a poor prognosis in pancreatic cancer patients. Herein we report a complete contig of human bacterial artificial chromosome (BAC) clones covering the deleted region. The region was covered by 21 BAC clones in a minimum tiling path. The clones were confirmed to exist at 12q21 by fluorescence In situ hybridization. We identified novel 40 sequence tagged sites and mapped 10 expressed sequence tags in this region. The BAC contig reported here provides an avenue for determining the complete nucleotide sequence and mining putative tumor suppressor genes in the deleted region of pancreatic cancer at 12q21.",

keywords = "12q21, bacterial artificial chromosome, expressed sequence tag, pancreatic cancer, physical map, sequence tagged site",

author = "Youssef, {Emile M.} and Kenzo Kaneko and Toshimasa Yatsuoka and Yutaka Hayashi and Masato Hoshi and Akira Horii and Toru Furukawa",

note = "Funding Information: We are grateful to Dr. Barbara Lee Smith Pierce (the Life Science Coordinator for the University of Maryland Asian Division) for editorial work in the preparation of this manuscript. We are also grateful to Naomi Kanai for technical assistance. This work was supported by the Japanese Ministries of Education, Science, Sports and Culture, and Health and Welfare and the Vehicle Racing Commemorative Foundation.",

year = "2001",

doi = "10.3109/10425170109041339",

language = "English",

volume = "11",

pages = "541--546",

journal = "Mitochondrial DNA",

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TY - JOUR

T1 - Human BAC contig covering the deleted region in pancreatic cancer at 12q21

AU - Youssef, Emile M.

AU - Kaneko, Kenzo

AU - Yatsuoka, Toshimasa

AU - Hayashi, Yutaka

AU - Hoshi, Masato

AU - Horii, Akira

AU - Furukawa, Toru

N1 - Funding Information: We are grateful to Dr. Barbara Lee Smith Pierce (the Life Science Coordinator for the University of Maryland Asian Division) for editorial work in the preparation of this manuscript. We are also grateful to Naomi Kanai for technical assistance. This work was supported by the Japanese Ministries of Education, Science, Sports and Culture, and Health and Welfare and the Vehicle Racing Commemorative Foundation.

PY - 2001

Y1 - 2001

N2 - In sporadic human primary pancreatic cancer tissues, loss of heterozygosity is frequently observed in the 1-cM region between D12S81 and D12S1719 at 12q21. Loss of this chromosome arm is known to be associate with a poor prognosis in pancreatic cancer patients. Herein we report a complete contig of human bacterial artificial chromosome (BAC) clones covering the deleted region. The region was covered by 21 BAC clones in a minimum tiling path. The clones were confirmed to exist at 12q21 by fluorescence In situ hybridization. We identified novel 40 sequence tagged sites and mapped 10 expressed sequence tags in this region. The BAC contig reported here provides an avenue for determining the complete nucleotide sequence and mining putative tumor suppressor genes in the deleted region of pancreatic cancer at 12q21.

AB - In sporadic human primary pancreatic cancer tissues, loss of heterozygosity is frequently observed in the 1-cM region between D12S81 and D12S1719 at 12q21. Loss of this chromosome arm is known to be associate with a poor prognosis in pancreatic cancer patients. Herein we report a complete contig of human bacterial artificial chromosome (BAC) clones covering the deleted region. The region was covered by 21 BAC clones in a minimum tiling path. The clones were confirmed to exist at 12q21 by fluorescence In situ hybridization. We identified novel 40 sequence tagged sites and mapped 10 expressed sequence tags in this region. The BAC contig reported here provides an avenue for determining the complete nucleotide sequence and mining putative tumor suppressor genes in the deleted region of pancreatic cancer at 12q21.

KW - 12q21

KW - bacterial artificial chromosome

KW - expressed sequence tag

KW - pancreatic cancer

KW - physical map

KW - sequence tagged site

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U2 - 10.3109/10425170109041339

DO - 10.3109/10425170109041339

M3 - Article

C2 - 11696982

AN - SCOPUS:0034886170

SN - 1940-1736

VL - 11

SP - 541

EP - 546

JO - Mitochondrial DNA

JF - Mitochondrial DNA

IS - 6

ER -

Human BAC contig covering the deleted region in pancreatic cancer at 12q21

Abstract

Keywords

UN SDGs

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