TY - JOUR
T1 - Human GLUT-2 overexpression does not affect glucose-stimulated insulin secretion in MIN6 cells
AU - Ishihara, H.
AU - Asano, T.
AU - Tsukuda, K.
AU - Katagiri, H.
AU - Inukai, K.
AU - Anai, M.
AU - Yazaki, Y.
AU - Miyazaki, J. I.
AU - Kikuchi, M.
AU - Oka, Y.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Accumulated evidence suggests that GLUT-2, in addition to its role in glucose transport, may also have other functions in glucose-stimulated insulin secretion. As a first step in addressing this possibility, we have engineered MIN6 cells overexpressing human GLUT-2 by transfection with human GLUT-2 cDNA. Stable transformants harboring human GLUT-2 cDNA exhibited an approximately twofold increase in 3-O-methyl-D-glucose uptake at 0.5 and 15 mM. Glucokinase activity or glucose utilization measured by conversion of [5- 3H]glucose to [3H]H2O was not, however, altered in the MIN6 cells overexpressing human GLUT-2. Furthermore, glucose-stimulated insulin secretion was not affected by overexpression of human GLUT-2. An abundance of GLUT-2, therefore, does not correlate with the glucose responsiveness of cells in which glycolysis is regulated at the glucose phosphorylating step. These data suggest that GLUT-2 by itself does not have significant functions other than its role in glucose transport in glucose sensing by MIN6 cells.
AB - Accumulated evidence suggests that GLUT-2, in addition to its role in glucose transport, may also have other functions in glucose-stimulated insulin secretion. As a first step in addressing this possibility, we have engineered MIN6 cells overexpressing human GLUT-2 by transfection with human GLUT-2 cDNA. Stable transformants harboring human GLUT-2 cDNA exhibited an approximately twofold increase in 3-O-methyl-D-glucose uptake at 0.5 and 15 mM. Glucokinase activity or glucose utilization measured by conversion of [5- 3H]glucose to [3H]H2O was not, however, altered in the MIN6 cells overexpressing human GLUT-2. Furthermore, glucose-stimulated insulin secretion was not affected by overexpression of human GLUT-2. An abundance of GLUT-2, therefore, does not correlate with the glucose responsiveness of cells in which glycolysis is regulated at the glucose phosphorylating step. These data suggest that GLUT-2 by itself does not have significant functions other than its role in glucose transport in glucose sensing by MIN6 cells.
KW - glucose metabolism
KW - glucose sensing
KW - pancreatic β-cells
KW - β-cell lines
UR - http://www.scopus.com/inward/record.url?scp=0029199814&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029199814&partnerID=8YFLogxK
U2 - 10.1152/ajpendo.1995.269.5.e897
DO - 10.1152/ajpendo.1995.269.5.e897
M3 - Article
C2 - 7491941
AN - SCOPUS:0029199814
SN - 0002-9513
VL - 269
SP - E897-E902
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
IS - 5 32-5
ER -
