HVJ-E/importin-β hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery

Takeshi Kawazu; Hiroyuki Kanzaki; Atsushi Uno; Hideki Azuma; Takeshi Nagasaki

doi:10.1016/j.biopha.2012.02.005

HVJ-E/importin-β hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery

Takeshi Kawazu, Hiroyuki Kanzaki, Atsushi Uno, Hideki Azuma, Takeshi Nagasaki

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

In order to enhance the nuclear import of the transgene, we prepared plasmid DNA/importin-β conjugates consisting of biotinylated poly(ethylenimine)s and recombinant streptavidin-fused importin-β. Hemagglutinating virus of Japan-envelope vector containing the PEI polyplex/importin-β conjugate showed high transfection efficiency not only in vitro but also in vivo. We showed that novel HVJ-E/importin-β-conjugated PEI polyplex hybrid vector could overcome plasma and nuclear membrane barriers to achieve effective transfection.

Original language	English
Pages (from-to)	519-524
Number of pages	6
Journal	Biomedicine and Pharmacotherapy
Volume	66
Issue number	7
DOIs	https://doi.org/10.1016/j.biopha.2012.02.005
Publication status	Published - 2012 Oct
Externally published	Yes

Keywords

Gene delivery
Hemagglutinating virus of Japan
Importin
Nuclear import
Poly(ethyleneimine)s

ASJC Scopus subject areas

Pharmacology

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10.1016/j.biopha.2012.02.005

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title = "HVJ-E/importin-β hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery",

abstract = "In order to enhance the nuclear import of the transgene, we prepared plasmid DNA/importin-β conjugates consisting of biotinylated poly(ethylenimine)s and recombinant streptavidin-fused importin-β. Hemagglutinating virus of Japan-envelope vector containing the PEI polyplex/importin-β conjugate showed high transfection efficiency not only in vitro but also in vivo. We showed that novel HVJ-E/importin-β-conjugated PEI polyplex hybrid vector could overcome plasma and nuclear membrane barriers to achieve effective transfection.",

keywords = "Gene delivery, Hemagglutinating virus of Japan, Importin, Nuclear import, Poly(ethyleneimine)s",

author = "Takeshi Kawazu and Hiroyuki Kanzaki and Atsushi Uno and Hideki Azuma and Takeshi Nagasaki",

note = "Funding Information: This work was supported by SORST program in Japan Science and Technology Corporation (JST) and a Grant-in-Aid for Scientific Research (C) (No. 17550159) from Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Prof. Housaka (Japan Advanced Institute of Science and Technology) for the gift of pGSH plasmid.",

year = "2012",

month = oct,

doi = "10.1016/j.biopha.2012.02.005",

language = "English",

volume = "66",

pages = "519--524",

journal = "Biomedicine and Pharmacotherapy",

issn = "0753-3322",

publisher = "Elsevier Masson",

number = "7",

}

TY - JOUR

T1 - HVJ-E/importin-β hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery

AU - Kawazu, Takeshi

AU - Kanzaki, Hiroyuki

AU - Uno, Atsushi

AU - Azuma, Hideki

AU - Nagasaki, Takeshi

N1 - Funding Information: This work was supported by SORST program in Japan Science and Technology Corporation (JST) and a Grant-in-Aid for Scientific Research (C) (No. 17550159) from Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Prof. Housaka (Japan Advanced Institute of Science and Technology) for the gift of pGSH plasmid.

PY - 2012/10

Y1 - 2012/10

N2 - In order to enhance the nuclear import of the transgene, we prepared plasmid DNA/importin-β conjugates consisting of biotinylated poly(ethylenimine)s and recombinant streptavidin-fused importin-β. Hemagglutinating virus of Japan-envelope vector containing the PEI polyplex/importin-β conjugate showed high transfection efficiency not only in vitro but also in vivo. We showed that novel HVJ-E/importin-β-conjugated PEI polyplex hybrid vector could overcome plasma and nuclear membrane barriers to achieve effective transfection.

AB - In order to enhance the nuclear import of the transgene, we prepared plasmid DNA/importin-β conjugates consisting of biotinylated poly(ethylenimine)s and recombinant streptavidin-fused importin-β. Hemagglutinating virus of Japan-envelope vector containing the PEI polyplex/importin-β conjugate showed high transfection efficiency not only in vitro but also in vivo. We showed that novel HVJ-E/importin-β-conjugated PEI polyplex hybrid vector could overcome plasma and nuclear membrane barriers to achieve effective transfection.

KW - Gene delivery

KW - Hemagglutinating virus of Japan

KW - Importin

KW - Nuclear import

KW - Poly(ethyleneimine)s

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U2 - 10.1016/j.biopha.2012.02.005

DO - 10.1016/j.biopha.2012.02.005

M3 - Article

C2 - 22727893

AN - SCOPUS:84867337397

SN - 0753-3322

VL - 66

SP - 519

EP - 524

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

IS - 7

ER -

HVJ-E/importin-β hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery

Abstract

Keywords

ASJC Scopus subject areas

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