Hydrogel-based bioassay sheets for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells

Kuniaki Nagamine; Kohei Okamoto; Shingo Otani; Hirokazu Kaji; Makoto Kanzaki; Matsuhiko Nishizawa

doi:10.1039/c3bm60179j

Hydrogel-based bioassay sheets for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells

Kuniaki Nagamine, Kohei Okamoto, Shingo Otani, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa

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8 Citations (Scopus)

Abstract

Two types of hydrogel-based bioassay sheets were developed for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells: one is an oxygen sensor sheet and the other is an immunocapture sheet for a myokine, interleukin-6 (IL-6). These soft, molecularly permeable hydrogel-based bioassay sheets were directly laminated to another hydrogel on which myotubes were micropatterned, and displayed usefully measurable changes in local oxygen consumption and IL-6 secretion of myotubes upon electrically-induced contraction.

Original language	English
Pages (from-to)	252-256
Number of pages	5
Journal	Biomaterials Science
Volume	2
Issue number	2
DOIs	https://doi.org/10.1039/c3bm60179j
Publication status	Published - 2014 Feb

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abstract = "Two types of hydrogel-based bioassay sheets were developed for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells: one is an oxygen sensor sheet and the other is an immunocapture sheet for a myokine, interleukin-6 (IL-6). These soft, molecularly permeable hydrogel-based bioassay sheets were directly laminated to another hydrogel on which myotubes were micropatterned, and displayed usefully measurable changes in local oxygen consumption and IL-6 secretion of myotubes upon electrically-induced contraction.",

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AU - Nagamine, Kuniaki

AU - Okamoto, Kohei

AU - Otani, Shingo

AU - Kaji, Hirokazu

AU - Kanzaki, Makoto

AU - Nishizawa, Matsuhiko

PY - 2014/2

Y1 - 2014/2

N2 - Two types of hydrogel-based bioassay sheets were developed for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells: one is an oxygen sensor sheet and the other is an immunocapture sheet for a myokine, interleukin-6 (IL-6). These soft, molecularly permeable hydrogel-based bioassay sheets were directly laminated to another hydrogel on which myotubes were micropatterned, and displayed usefully measurable changes in local oxygen consumption and IL-6 secretion of myotubes upon electrically-induced contraction.

AB - Two types of hydrogel-based bioassay sheets were developed for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells: one is an oxygen sensor sheet and the other is an immunocapture sheet for a myokine, interleukin-6 (IL-6). These soft, molecularly permeable hydrogel-based bioassay sheets were directly laminated to another hydrogel on which myotubes were micropatterned, and displayed usefully measurable changes in local oxygen consumption and IL-6 secretion of myotubes upon electrically-induced contraction.

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Hydrogel-based bioassay sheets for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells

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