Hydrogel-based imaging sensor for the assay of exercise-dependent metabolic regulation in skeletal muscle cells

Kuniaki Nagamine, Kohei Okamoto, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Two types of hydrogel-based metabolite-responsive imaging sensor sheets were developed for in vitro assay of contraction-dependent metabolic regulation in skeletal muscle cells: one is an oxygen sensor and the other is an Interleukin-6 (IL-6) sensor. These soft, molecularly permeable hydrogel-based sensors were directly laminated to another hydrogel on which myotubes were micropatterned, and displayed usefully measureable changes in local oxygen consumption and IL-6 secretion activity of myotubes upon electrically-induced contraction.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1505-1507
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • Myokine
  • Oxygen sensor
  • Skeletal muscle cell

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