TY - GEN
T1 - Hydrogel culture dish integrated with organic electrodes for efficient stimulation of hiPSC-derived cardiomyocytes
AU - Sumomozawa, Kensuke
AU - Nagamine, Kuniaki
AU - Kai, Hiroyuki
AU - Yoshida, Shotaro
AU - Kaji, Hirokazu
AU - Nishizawa, Matsuhiko
N1 - Funding Information:
This work was partly supported by MIRAI and Center of Innovation Program (COI-Stream) from Japan Science and Technology Agency (JST) and Grand-in-Aid for Scientific Research A (25246016) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
PY - 2018
Y1 - 2018
N2 - We propose a hydrogel-based bioreactor integrated with organic electrodes for efficient electrical stimulation of cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs). The bioreactor was fabricated by integration of poly(3,4-ethylenedioxythiophene) (PEDOT)/polyurethane (PU) electrodes and a hydrogel with a cell-positioning chamber. The fabricated organic electrodes had a high capacity compared to conventional metal electrodes that prevented unfavorable electric polarization. Cardiomyocytes derived from hiPSCs were cultured in the bioreactor, and their beating frequency was synchronized to frequency of electrical stimulation applied by the electrodes. Efficient electrical stimulation was achieved by optimizing configuration of the electrodes.
AB - We propose a hydrogel-based bioreactor integrated with organic electrodes for efficient electrical stimulation of cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs). The bioreactor was fabricated by integration of poly(3,4-ethylenedioxythiophene) (PEDOT)/polyurethane (PU) electrodes and a hydrogel with a cell-positioning chamber. The fabricated organic electrodes had a high capacity compared to conventional metal electrodes that prevented unfavorable electric polarization. Cardiomyocytes derived from hiPSCs were cultured in the bioreactor, and their beating frequency was synchronized to frequency of electrical stimulation applied by the electrodes. Efficient electrical stimulation was achieved by optimizing configuration of the electrodes.
KW - Cardiomyocyte
KW - Hydrogel
KW - IPSC
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M3 - Conference contribution
AN - SCOPUS:85079816546
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1445
EP - 1447
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -