TY - GEN
T1 - Hydrogel-supported skeletal muscle cell-based bioassay system
AU - Nagamine, Kuniaki
AU - Otani, Shingo
AU - Takeda, Mai
AU - Kanzaki, Makoto
AU - Nishizawa, Matsuhiko
PY - 2011
Y1 - 2011
N2 - Contractile C2C12 myotube line patterns supported by a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. Myotube line patterns cultured on dish were transferred with 100% efficiency to the surface of fibrin gel sheets. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. The gel sheet-supported C 2C12 myotube micropatterns were combined with a microelectrode array chip to fabricate a skeletal muscle cell-based bioassay system. The contractile behavior of each myotube line pattern on the gel was individually controlled by localized electrical stimulation using microelectrode arrays that had been previously modified with the electropolymerized conducting polymer. We successfully demonstrated fluorescent imaging of the contraction-induced translocation of the glucose transporter, GLUT4, from intracellular vesicles to the plasma membrane of the myotubes. This device is applicable for the bioassay of contraction-induced metabolic alterations in a skeletal muscle cell.
AB - Contractile C2C12 myotube line patterns supported by a fibrin gel have been developed to afford a physiologically relevant and stable bioassay system. Myotube line patterns cultured on dish were transferred with 100% efficiency to the surface of fibrin gel sheets. We found that the myotubes supported by an elastic fibrin gel maintained their line patterns and contractile activities for a longer period of time (one week) than myotubes adhered on a conventional culture dish. The gel sheet-supported C 2C12 myotube micropatterns were combined with a microelectrode array chip to fabricate a skeletal muscle cell-based bioassay system. The contractile behavior of each myotube line pattern on the gel was individually controlled by localized electrical stimulation using microelectrode arrays that had been previously modified with the electropolymerized conducting polymer. We successfully demonstrated fluorescent imaging of the contraction-induced translocation of the glucose transporter, GLUT4, from intracellular vesicles to the plasma membrane of the myotubes. This device is applicable for the bioassay of contraction-induced metabolic alterations in a skeletal muscle cell.
UR - http://www.scopus.com/inward/record.url?scp=84863300896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863300896&partnerID=8YFLogxK
U2 - 10.1109/MHS.2011.6102223
DO - 10.1109/MHS.2011.6102223
M3 - Conference contribution
AN - SCOPUS:84863300896
SN - 9781457713613
T3 - 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
SP - 180
EP - 185
BT - 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
PB - IEEE Computer Society
T2 - 22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
Y2 - 6 November 2011 through 9 November 2011
ER -