TY - JOUR
T1 - Hydrolysis of plasmalogen by phospholipase A1 from Streptomyces albidoflavus for early detection of dementia and arteriosclerosis
AU - Sakasegawa, Shin ich
AU - Maeba, Ryota
AU - Murayama, Kazutaka
AU - Matsumoto, Hideyuki
AU - Sugimori, Daisuke
N1 - Funding Information:
This work was supported in part by JSPS KAKENHI Grant Number 15K05557, a grant from the Adaptable and Seamless Technology Transfer Program through target-driven R&D, JST (Grant No. AS251Z00099P), a grant from Research for Promoting Technological Seeds” from Innovation Plaza Miyagi, Japan Science and Technology Agency, and also by a research grant funding from the Takahashi Industrial and Economic Research Foundation to D. S.
Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Objectives: To obtain an ethanolamine plasmalogen (PlsEtn)-hydrolyzing enzyme and to develop an assay that would help determine PlsEtn concentrations in human serum as an indicator of Alzheimer-type dementia and of arteriosclerosis. Results: Phospholipase A1s, SaPLA1 and SvPLA1 from, respectively, Streptomyces albidoflavus NA297 and S. avermitilis JCM5070—but not phospholipase B from Streptomyces sp. NA684, PLA2-Nagase from S. avermitilis, PLA2IIL from S. violaceoruber nor LIPOMOD 699L (porcine phospholipase)—hydrolyzed choline plasmalogen (PlsCho) and PlsEtn (PlsCho preferred over PlsEtn). Using a combination of SaPLA1, lysoplasmalogen-specific phospholipase D (LyPls-PLD), with amine oxidase, an end-point assay was developed for measuring serum PlsEtn concentration. The standard curve, generated using various amounts of PlsEtn in this assay, was linear between 0 and 0.2 mM. PlsEtn concentrations in forty-seven serum samples, determined independently by this enzyme-based assay and 125I-HPLC method, exhibited a linear relationship, indicating that the assay is suitable for fast and accurate measurement of serum PlsEtn concentration. Conclusions: An assay, developed using SaPLA1, LyPls-PLD, and AOX, selectively measured PlsEtn levels in blood samples. This assay could be a useful diagnostic tool for early stage detection of diseases such as Alzheimer-type dementia and arteriosclerosis.
AB - Objectives: To obtain an ethanolamine plasmalogen (PlsEtn)-hydrolyzing enzyme and to develop an assay that would help determine PlsEtn concentrations in human serum as an indicator of Alzheimer-type dementia and of arteriosclerosis. Results: Phospholipase A1s, SaPLA1 and SvPLA1 from, respectively, Streptomyces albidoflavus NA297 and S. avermitilis JCM5070—but not phospholipase B from Streptomyces sp. NA684, PLA2-Nagase from S. avermitilis, PLA2IIL from S. violaceoruber nor LIPOMOD 699L (porcine phospholipase)—hydrolyzed choline plasmalogen (PlsCho) and PlsEtn (PlsCho preferred over PlsEtn). Using a combination of SaPLA1, lysoplasmalogen-specific phospholipase D (LyPls-PLD), with amine oxidase, an end-point assay was developed for measuring serum PlsEtn concentration. The standard curve, generated using various amounts of PlsEtn in this assay, was linear between 0 and 0.2 mM. PlsEtn concentrations in forty-seven serum samples, determined independently by this enzyme-based assay and 125I-HPLC method, exhibited a linear relationship, indicating that the assay is suitable for fast and accurate measurement of serum PlsEtn concentration. Conclusions: An assay, developed using SaPLA1, LyPls-PLD, and AOX, selectively measured PlsEtn levels in blood samples. This assay could be a useful diagnostic tool for early stage detection of diseases such as Alzheimer-type dementia and arteriosclerosis.
KW - Arteriosclerosis
KW - Dementia: diagnostic enzyme
KW - Phospholipase A1
KW - Plasmalogen
KW - Plasmalogen determination
KW - Streptomyces
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U2 - 10.1007/s10529-015-1955-5
DO - 10.1007/s10529-015-1955-5
M3 - Article
C2 - 26354853
AN - SCOPUS:84953636820
SN - 0141-5492
VL - 38
SP - 109
EP - 116
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 1
ER -
