Hydrolysis of s-2-aminoethylcysteinyl peptide bond by achromobacter protease i

Takeharu Masaki, Toshiyuki Takiya, Susumu Tsunasawa, Shigefumi Kuwahara, Fumio Sakiyama, Masami Soejima

Research output: Contribution to journalArticlepeer-review


The substrate specificity of Achromobacter protease I (API) was examined for S-2-aminoethyl(AE)cysteinyl bonds in Bz-AEC-OMe/OEt, Bz-AEC-NH2, and AE-insulin B chain. The protease hydrolyzed all of the tested AE-cysteinyl bonds at the same rate as that of lysyl bonds. Kinetic parameters were estimated for this hydrolysis reaction.

Original languageEnglish
Pages (from-to)215-216
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Issue number1
Publication statusPublished - 1994 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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