Hydroxyethyl cellulose matrix applied to serial crystallography

Michihiro Sugahara; Takanori Nakane; Tetsuya Masuda; Mamoru Suzuki; Shigeyuki Inoue; Changyong Song; Rie Tanaka; Toru Nakatsu; Eiichi Mizohata; Fumiaki Yumoto; Kensuke Tono; Yasumasa Joti; Takashi Kameshima; Takaki Hatsui; Makina Yabashi; Osamu Nureki; Keiji Numata; Eriko Nango; So Iwata

doi:10.1038/s41598-017-00761-0

Hydroxyethyl cellulose matrix applied to serial crystallography

Michihiro Sugahara, Takanori Nakane, Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Rie Tanaka, Toru Nakatsu, Eiichi Mizohata, Fumiaki Yumoto, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, Eriko Nango, So Iwata

IMRAM - Division of Organic- and Biomaterials Research

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images.

Original language	English
Article number	703
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-00761-0
Publication status	Published - 2017 Dec 1

Access to Document

10.1038/s41598-017-00761-0

Cite this

Sugahara, M., Nakane, T., Masuda, T., Suzuki, M., Inoue, S., Song, C., Tanaka, R., Nakatsu, T., Mizohata, E., Yumoto, F., Tono, K., Joti, Y., Kameshima, T., Hatsui, T., Yabashi, M., Nureki, O., Numata, K., Nango, E., & Iwata, S. (2017). Hydroxyethyl cellulose matrix applied to serial crystallography. Scientific Reports, 7(1), Article 703. https://doi.org/10.1038/s41598-017-00761-0

@article{7ba47944f15e4e3f84c98f51fe9c4d8b,

title = "Hydroxyethyl cellulose matrix applied to serial crystallography",

abstract = "Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images.",

author = "Michihiro Sugahara and Takanori Nakane and Tetsuya Masuda and Mamoru Suzuki and Shigeyuki Inoue and Changyong Song and Rie Tanaka and Toru Nakatsu and Eiichi Mizohata and Fumiaki Yumoto and Kensuke Tono and Yasumasa Joti and Takashi Kameshima and Takaki Hatsui and Makina Yabashi and Osamu Nureki and Keiji Numata and Eriko Nango and So Iwata",

note = "Funding Information: National Research Foundation of Korea (grants NRF-2015R1A5A1009962 and NRF-2016R1A2B3010980) Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = dec,

day = "1",

doi = "10.1038/s41598-017-00761-0",

language = "English",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Hydroxyethyl cellulose matrix applied to serial crystallography

AU - Sugahara, Michihiro

AU - Nakane, Takanori

AU - Masuda, Tetsuya

AU - Suzuki, Mamoru

AU - Inoue, Shigeyuki

AU - Song, Changyong

AU - Tanaka, Rie

AU - Nakatsu, Toru

AU - Mizohata, Eiichi

AU - Yumoto, Fumiaki

AU - Tono, Kensuke

AU - Joti, Yasumasa

AU - Kameshima, Takashi

AU - Hatsui, Takaki

AU - Yabashi, Makina

AU - Nureki, Osamu

AU - Numata, Keiji

AU - Nango, Eriko

AU - Iwata, So

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images.

AB - Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images.

UR - http://www.scopus.com/inward/record.url?scp=85018432559&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018432559&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-00761-0

DO - 10.1038/s41598-017-00761-0

M3 - Article

C2 - 28386083

AN - SCOPUS:85018432559

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 703

ER -

Hydroxyethyl cellulose matrix applied to serial crystallography

Abstract

Access to Document

Other files and links

Fingerprint

Cite this