TY - JOUR
T1 - Identification and characterization of 12 tetranucleotide microsatellite markers in the white-spotted char Salvelinus leucomaenis
AU - Yamaguchi, Kohtaroh
AU - Saito, Masataka
AU - Nakajima, Masamichi
N1 - Funding Information:
We would like to thank the Chichibu Fishermen’s Cooperative Association, Susumu Yamanaka, Daisei Ando, Tadashi Okura, Yoshinari Otomo, Masashi Mizuochi, Shigeru Kibe, Hiromasa Yamaguchi, and Nobuhiko Taniguchi for their aid in sample collections and their suggestions. We would also like to thank Mariko Miyazaki for the laboratory assistance. This work was partly supported by JSPS Grants-in-Aid for Scientific Research, grant no. 25252035, and the Research Foundation of the Institute of Nutrition Sciences, Kagawa Nutrition University.
Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - The white-spotted char (Salvelinus leucomaenis) is an important species for Japanese fisheries, with a biogeographic range from Kamchatka in the Russian Federation to the Chugoku district in Japan. The population is decreasing owing to environmental changes and fishing pressure. Therefore, detailed information on this species is required to develop effective conservation strategies and harvesting practices. To provide tools for addressing the ecology and management for this species, we developed 12 polymorphic tetranucleotide microsatellite markers, which were optimized into four multiplexes. No null alleles were detected at any locus. The number of alleles per locus, and observed and expected heterozygosities across two populations were 1–19, 0.000–1.000, and 0.000–0.938, respectively. No significant linkage disequilibrium or departure from Hardy–Weinberg equilibrium was detected. These loci will be useful for conducting population genetics studies in this species.
AB - The white-spotted char (Salvelinus leucomaenis) is an important species for Japanese fisheries, with a biogeographic range from Kamchatka in the Russian Federation to the Chugoku district in Japan. The population is decreasing owing to environmental changes and fishing pressure. Therefore, detailed information on this species is required to develop effective conservation strategies and harvesting practices. To provide tools for addressing the ecology and management for this species, we developed 12 polymorphic tetranucleotide microsatellite markers, which were optimized into four multiplexes. No null alleles were detected at any locus. The number of alleles per locus, and observed and expected heterozygosities across two populations were 1–19, 0.000–1.000, and 0.000–0.938, respectively. No significant linkage disequilibrium or departure from Hardy–Weinberg equilibrium was detected. These loci will be useful for conducting population genetics studies in this species.
KW - Genetic variation
KW - Next-generation sequencing
KW - Salvelinus leucomaenis
KW - Tetranucleotide microsatellites
KW - White-spotted char
UR - http://www.scopus.com/inward/record.url?scp=84928991327&partnerID=8YFLogxK
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U2 - 10.1007/s12686-014-0405-3
DO - 10.1007/s12686-014-0405-3
M3 - Letter
AN - SCOPUS:84928991327
SN - 1877-7252
VL - 7
SP - 497
EP - 499
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -