TY - JOUR
T1 - Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe
AU - Sato, Shusei
AU - Suzuki, Hideki
AU - Widyastuti, Utut
AU - Hotta, Yasuo
AU - Tabata, Satoshi
PY - 1994/7
Y1 - 1994/7
N2 - Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA prmary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation.
AB - Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA prmary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation.
KW - Schizosaccharomyces pombe
KW - Sexual differentiation
KW - Stage-specific genes
KW - Subtractive screening
UR - http://www.scopus.com/inward/record.url?scp=0028199914&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028199914&partnerID=8YFLogxK
U2 - 10.1007/BF00326301
DO - 10.1007/BF00326301
M3 - Article
C2 - 7954893
AN - SCOPUS:0028199914
SN - 0172-8083
VL - 26
SP - 31
EP - 37
JO - Current Genetics
JF - Current Genetics
IS - 1
ER -